Phospho-PPAR- Gamma (Ser112) Rabbit Polyclonal Antibody#abs130911

abs130911-50ug

1-2 Weeks

201

abs130911-100ug

1-2 Weeks

301

Description

The nuclear receptor gene in question encodes a protein belonging to the peroxisome proliferator-activated receptor (PPAR) subfamily. PPARs collaborate with retinoid X receptors (RXRs) to form heterodimers, which are responsible for regulating the transcription of various genes. Within the PPAR subfamily, there are three known subtypes: PPAR-alpha, PPAR-delta, and PPAR-gamma. Please note that the generated content is based on the original text information, but it has been rearranged to provide a highly similar alternative.

Other names

PPAR gamma, also known as Peroxisome Proliferator Activated Receptor gamma or Nuclear receptor subfamily 1 group C member 3, is a type of nuclear receptor that functions in various biological processes, including glucose and lipid metabolism, adipocyte differentiation, and inflammation. It is encoded by the PPARG gene, which has two main isoforms called PPARG1 and PPARG2.
In addition to its role in regulating metabolic homeostasis, PPAR gamma has been implicated in the development and progression of several diseases, including type 2 diabetes, obesity, and cancer. Its activation by natural ligands, such as certain polyunsaturated fatty acids, can improve insulin sensitivity and lipid profiles, while synthetic agonists, such as thiazolidinediones, have been used as insulin sensitizers in diabetic patients.
Despite its beneficial effects, PPAR gamma has also been associated with adverse events, such as increased risk of heart failure and bone fractures, which have limited its clinical use. Nevertheless, research continues to uncover new information about this important transcription factor and its potential therapeutic applications.

Source

Rabbit

Specificity

The Phospho-PPAR-gamma (Ser112) Antibody specifically identifies PPAR-gamma protein levels that are naturally present in the body and are phosphorylated at Serine 112. To clarify, this antibody specifically detects the phosphorylated form of PPAR-gamma at Serine 112.

Predictive reaction species

Rabbit;Dog;Horse

Application

The recommended dilutions for using this antibody are as follows: 1:500-1:2000 for Western Blotting, 1:50-1:200 for Immunohistochemistry, 1:200 for Immunofluorescence, and 1:20000-1:40000 for ELISA assays using peptides. These dilution ratios are based on the original text information and are highly similar to the information provided in the original content, although arranged in a different order.

A peptide synthesized from the human PPAR-gamma, specifically targeting the phosphorylation site of Serine 112, has been developed. The peptide is designed to closely resemble the original human PPAR-gamma sequence around the Serine 112 phosphorylation site. Its synthesis ensures a high level of similarity to the target region, allowing for specific interactions and potential modulations of PPAR-gamma activity. This synthesized peptide offers a valuable tool for investigating the role of Serine 112 phosphorylation in PPAR-gamma function and related signaling pathways.

Properties

1mg/ml

Purification

The serum from rabbits was first purified, and the resulting antibody was obtained through sequential chromatography using columns specifically designed for phospho- and non-phospho-peptide affinity. To create a highly similar content by rearranging the original text, we can say:
Sequential chromatography was conducted on affinity columns, including both phospho- and non-phospho-peptide columns, to purify the antibody from rabbit serum. This process involved the initial purification of the serum, followed by affinity purification to isolate the desired antibody.

Clonality

Polyclonal Antibody

To ensure the longevity of the product, it is advised to store it at a temperature of -20 °C for a year. It is important to avoid repeated freeze/thaw cycles as it may compromise the integrity of the product. Following these guidelines will ensure the quality and efficacy of the product for an extended period of time.

Storage buffer

PBS, pH 7.4,50% glycerol.

Target

Background

 Nuclear receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the nuclear receptor binds to DNA specific PPAR response elements (PPRE) and modulates the transcription of its target genes, such as acyl-CoA oxidase. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis. ARF6 acts as a key regulator of the tissue-specific adipocyte P2 (aP2) enhancer. Acts as a critical regulator of gut homeostasis by suppressing NF-kappa-B-mediated proinflammatory responses. Plays a role in the regulation of cardiovascular circadian rhythms by regulating the transcription of ARNTL/BMAL1 in the blood vessels (By similarity).

Tissue specificity

Highest expression in adipose tissue. Lower in skeletal muscle, spleen, heart and liver. Also detectable in placenta, lung and ovary.

Celluar localization

Cytosol;Nucleus;

P37231