Phospho-I KappaB- Alpha (Ser32/Ser36) Rabbit Polyclonal Antibody#abs130622

abs130622-50ug

1-2 Weeks

201

abs130622-100ug

1-2 Weeks

301

Description

The NFKB complex is formed by the binding of either NFKB1 (MIM 164011) or NFKB2 (MIM 164012) with REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758). In order to keep NF-kappa-B inactive and confined to the cytoplasm, I-kappa-B proteins (NFKBIA or NFKBIB, MIM 604495) play a crucial role by inhibiting the NFKB complex. These I-kappa-B proteins trap NF-kappa-B, preventing its activation. Hence, the balance between NFKB1/NFKB2 and I-kappa-B proteins is vital for regulating the activity of NF-kappa-B.

Other names

There are several names for the protein that inhibits the nuclear factor kappa-light-chain-enhancer of activated B cells, including I kappa B alpha, IkappaBalpha, and IKBA. It is also known as NF-kappa-B inhibitor alpha, NFKBI, and NFKBIA. This protein plays an important role in regulating the immune response and preventing inappropriate activation of the NF-kappa-B pathway. Another name for I kappa B alpha is Major histocompatibility complex enhancer-binding protein MAD3. Overall, it is a critical component of the immune system, maintaining homeostasis by preventing excessive inflammation and cellular damage.

Source

Rabbit

Specificity

The Phospho-I kappaB-alpha (Ser32/Ser36) Antibody specifically targets the Serine 32/Serine 36-phosphorylated form of endogenous I kappaB-alpha. It is unable to detect non-phosphorylated I kappaB-alpha.

Predictive reaction species

Pig;Rabbit;Sheep;Dog;Bovine

Application

The recommended dilutions for using this antibody in various applications are as follows: for Western blotting, dilute at 1:500-1:2000; for immunohistochemistry, dilute at 1:50-1:500; for immunofluorescence or immunocytochemistry, dilute at 1:100-1:500; for ELISA using peptide, dilute at 1:20000-1:40000. You can use these dilutions as a guide to ensure optimal results when using this antibody in your experiments. By following these recommended dilutions, you can expect highly specific and sensitive detection of your target protein in your samples. So make sure to use this antibody in the recommended dilutions to achieve reliable and reproducible results.

A peptide that has been synthesized using human I kappaB- alpha, specifically around the phosphorylation site of Serine 32/Serine 36, has been created. To reiterate, this synthesized peptide is highly similar to the original human I kappaB- alpha protein and is being used for research purposes.

Properties

1mg/ml

Purification

The sequential chromatography on phospho- and non-phospho-peptide affinity columns was used to purify the antibody from rabbit serum, resulting in highly purified antibody.

Clonality

Polyclonal Antibody

To preserve the material's quality, it is recommended to store it at a temperature of minus 20 degrees Celsius for up to one year. It is important to avoid subjecting the sample to repeated cycles of freezing and thawing.

Storage buffer

This Rabbit IgG is suspended in a solution of phosphate buffered saline with a pH of 7.4 and contains 150 millimoles of NaCl, 0.02% sodium azide as a preservative, and 50% glycerol. It is recommended to store this product at a temperature of -20 °C for maximum stability, with a shelf life of up to 12 months from the date of receipt. We advise caution when handling this product, and recommend following proper laboratory protocols for best results.

Target

Background

 Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Tissue specificity

Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.

Celluar localization

Cytosol;Nucleus;Plasma Membrane;

P25963