Rabbit Anti-Nibrin Polyclonal Antibody #abs130579

abs130579-50ug

1-2 Weeks

201

abs130579-100ug

1-2 Weeks

301

Description

The NBS1 protein is a crucial component of the MRE11/RAD50 double-strand break repair complex, which is responsible for repairing DNA double-strand breaks and activating DNA damage-induced checkpoints. Patients with mutations in the NBS1 gene experience the Nijmegen breakage syndrome, which is a rare chromosomal instability syndrome inherited in an autosomal recessive pattern. It is important to understand the mechanisms behind NBS1 and its role in DNA repair and checkpoint activation to develop potential therapeutic approaches for Nijmegen breakage syndrome and other related disorders.

Other names

Nijmegen breakage syndrome 1 (nibrin), also known as NBN_HUMAN or NBS1, is a cell cycle regulatory protein involved in DNA repair. It is part of the MRE11/RAD50 complex, where it interacts with p95 protein. Other names for NBS1 include Nibrin, FLJ10155, and MGC87362. Its function is crucial in the Nijmegen breakage syndrome, a genetic disorder characterized by increased sensitivity to DNA-damaging agents and a high predisposition to cancer.

Source

Rabbit

Specificity

The detection of endogenous levels of Nibrin can be achieved with the use of Nibrin Antibody.

Species Reactivity

Human

Application

WB1:5001:2000，/。IF/ICC1:1001:500。，ELISA（）1:200001:40000。，。

Immunogen

A synthesized peptide derived from human Nibrin

Properties

Concentration

1mg/ml

Purification

Immunogen affinity purified

Clonality

Polyclonal Antibody

Stability & Storage

To maintain the quality of the product, it is recommended to keep it in a freezer at a temperature of -20°C and avoid exposing it to repeated freeze and thaw cycles. This storage method can be followed for up to one year to ensure that the product remains in good condition.

Storage buffer

This solution contains Rabbit IgG in a phosphate buffered saline solution. The pH is 7.4, and the solution also contains 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It should be stored at -20 °C for optimal stability. The shelf life of this solution is 12 months from the date of receipt.

Target

Background

The MRE11-RAD50-NBN (MRN complex) is an essential component involved in various cellular processes related to DNA damage response and chromosome stability. It plays a critical role in repairing double-strand breaks (DSBs), promoting DNA recombination, maintaining telomere integrity, controlling cell cycle checkpoints, and facilitating meiosis. The complex exerts its functions through multiple activities provided by its constituent proteins.
MRE11, one of the components of the complex, possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity. These enzymatic functions are crucial for processing DNA ends and facilitating repair processes. RAD50, another component, is thought to bind DNA ends and hold them in close proximity, aiding in efficient repair. NBN, the third component, serves as a modulator of the DNA damage signal sensing. It recruits several kinases from the PI3/PI4-kinase family, including ATM, ATR, and possibly DNA-PKcs, to the sites of DNA damage and activates their functions. Additionally, NBN interacts with histone H2AX to recruit MRE11 and RAD50 to the vicinity of DSBs.
NBN also plays a significant role in maintaining telomere length. It generates a 3' overhang, which acts as a primer for telomerase-mediated elongation of telomeres. Moreover, NBN is involved in controlling intra-S-phase checkpoint, and there is evidence suggesting its participation in the G1 and G2 cell cycle checkpoints. Overall, the functions of NBS1/MRN complex encompass sensing, transducing, and executing DNA damage signals, ensuring the preservation of DNA integrity and genomic stability.
Furthermore, the complex forms a partnership with RBBP8 to connect DNA double-strand break sensing with resection processes. It also has the potential to enhance AKT1 phosphorylation, possibly through its association with the mTORC2 complex. These roles highlight the diverse and crucial functions of the MRN complex in maintaining genome stability and facilitating various cellular processes.

Tissue specificity

Ubiquitous. Expressed at high levels in testis.

Posttranslational modification

Phosphorylated by ATM in response of ionizing radiation, and such phosphorylation is responsible intra-S phase checkpoint control and telomere maintenance.

Celluar localization

Cytosol;Nucleus;

UniPort

O60934