Rabbit Anti-HDAC9 Polyclonal Antibody #abs130118

abs130118-50ug

1-2 Weeks

201

abs130118-100ug

1-2 Weeks

301

Description

HDAC9 is a member of the histone deacetylase family, subfamily 2, which functions as a transcriptional regulator. This protein is responsible for the deacetylation of lysine residues on the N-terminal portion of core histones H2A, H2B, H3 and H4. Its role in transcriptional regulation and cell cycle progression make it an important player in developmental events. The activities of histone deacetylases are mediated by the formation of large multiprotein complexes.

Other names

7B HD; HD9; HD7; HDAC7B; HDAC HD7B; HDAC9; HDAC9B; HDAC9FL; HDAC HDAC 7; HDAC 9A; HDAC-related protein; HDAC-related protein; HDAC; HDAC7; HDAC9; HDAC9_HUMAN; HDAC9B; HDAC9FL; HDRP; Histone deacetylase 4/5 related protein; Histone deacetylase 7; Histone deacetylase 7B; Histone deacetylase 9; Histone deacetylase 9A; Histone deacetylase-related protein; Histone deacetylase 7 interacting transcription repressor MITR; MEF2 interacting transcription repressor protein; MEF2-interacting transcription repressor MITR; KIAA0744;. The above content contains various histone deacetylase (HDAC) molecules, including HDAC7, HDAC7B, HDAC9, HDAC9B, HDAC9FL, HDRP, and HDAC-related proteins. These molecules are involved in gene regulation by modifying histone acetylation levels and can interact with transcription factors like MEF2. Additionally, HDACs play roles in various biological processes and are potential therapeutic targets in diseases such as cancer and neurodegenerative disorders.

Source

Rabbit

Specificity

The total HDAC9 levels can be detected by using the HDAC9 antibody, which specifically targets the endogenous HDAC9 protein. Generating a similar content with a different structure could be as follows: By employing the HDAC9 antibody, one can effectively measure the overall amounts of HDAC9 protein present in a sample, thereby enabling the detection of endogenous HDAC9 levels.

Species Reactivity

Human

Application

To achieve the best results with this antibody, we recommend using it at a concentration of 1:500 to 1:3000 for Western blotting, 1:50 to 1:200 for immunohistochemistry, and 1:100 to 1:500 for immunofluorescence or immunocytochemistry. For ELISA using a peptide substrate, we suggest a dilution range of 1:20000 to 1:40000. Following these guidelines will allow you to obtain highly specific and reliable results with this antibody.

Immunogen

A synthesized peptide derived from human HDAC9.

Properties

Concentration

1mg/ml

Purification

SulfoLink™ Coupling Resin was utilized to purify the antiserum through peptide affinity chromatography.

Clonality

Polyclonal Antibody

Stability & Storage

To maintain optimal storage conditions, it is recommended to keep the product at a temperature of -20°C for a period of one year. Avoid exposing the product to repeated cycles of freezing and thawing as this can affect its stability and efficacy over time. To preserve the integrity of the product, ensure that it is stored in a well-ventilated and dry environment that is free from any sources of contamination. By following these simple storage guidelines, you can ensure that your product remains effective and reliable for its intended use.

Storage buffer

This is a solution of Rabbit IgG in phosphate buffered saline with a pH level of 7.4. It additionally contains 150mM NaCl, 0.02% sodium azide, and 50% glycerol. To preserve its quality, store it at -20 °C. This solution remains stable for 12 months from the date of receipt.

Target

Background

The N-terminal part of the core histones (H2A, H2B, H3, and H4) undergo deacetylation thanks to the activity of this enzyme. The resulting histone deacetylation marks are crucial for epigenetic repression and have a significant impact on transcriptional regulation, cell cycle progression, and developmental events. By repressing MEF2-dependent transcription, this enzyme further contributes to the fine-tuning of gene expression in cells.

Tissue specificity

Broadly expressed, with highest levels in brain, heart, muscle and testis. Isoform 3 is present in human bladder carcinoma cells (at protein level).

Posttranslational modification

Phosphorylated on Ser-220 and Ser-450; which promotes 14-3-3-binding, impairs interaction with MEF2, and antagonizes antimyogenic activity. Phosphorylated on Ser-240; which impairs nuclear accumulation (By similarity). Isoform 7 is phosphorylated on Tyr-1010. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.Sumoylated.

Celluar localization

Nucleus;

UniPort

Q9UKV0