Rabbit Anti-TRHR Polyclonal Antibody #abs130578
Please note that the price mentioned above is just for your reference. For detailed pricing information, please get in touch with our seller, Vecent. Data Examples Our experiment involved the analysis of COS7 cell lysate using a TRHR antibody through western blot. To generate a differentiated...
Description
Catalog-specification | Delivery time | USD price |
abs130578-50ug | 1-2 Weeks | 201 |
abs130578-100ug | 1-2 Weeks | 301 |
Please note that the price mentioned above is just for your reference. For detailed pricing information, please get in touch with our seller, Vecent.
Overview | |
Description | The TRHR receptor is a receptor that binds to thyrotropin-releasing hormone. It is primarily activated by G proteins, which then initiate a calcium second messenger system in the cell. This receptor is classified as a member of the G-protein coupled receptor 1 family. It is important to note that the information provided may have been sourced from UniProtKB. In order to generate highly similar content, the original text can be rearranged and rephrased without using ChapGPT or engaging in dialogue to ensure a unique presentation of the information. |
Other names | The Thyroliberin receptor is also known as the Thyrotropin Releasing Hormone Receptor or TRH-R. It plays a crucial role in the body's hormonal system, responding to the presence of Thyrotropin-releasing hormone. This receptor is highly important in the regulation of thyroid-stimulating hormone and plays a crucial role in maintaining the balance of thyroid function. In humans, it is known as TRFR_HUMAN or TRHR. Understanding the function of this receptor is vital in understanding the many functions of the thyroid and how it affects overall health. |
Source | Rabbit |
Specificity | TRHR Antibody detects endogenous levels of TRHR. |
Species Reactivity | Human;Mouse;Rat |
Predictive reaction species | Xenopus;Dog;Horse |
Application | ，。，1:5001:2000。/，1:1001:500。（ELISA），，1:200001:40000。。 |
Immunogen | A synthesized peptide derived from human TRHR. |
Properties | |
Concentration | 1mg/ml |
Purification | The SulfoLink™ Coupling Resin was utilized in the purification of the antiserum through peptide affinity chromatography. The resulting product was highly purified and ready for use in various applications. |
Clonality | Polyclonal Antibody |
Stability & Storage | To ensure the preservation of the product, it is recommended to store it at a temperature of -20 °C for a period of one year. It is important to avoid subjecting it to repeated cycles of freezing and thawing. |
Storage buffer | This product contains Rabbit IgG in a solution of phosphate buffered saline at pH 7.4, with 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It should be stored at -20 °C for best results and is stable for up to 12 months from the date of receipt. |
Target | |
Background | The activation of a phosphatidylinositol-calcium second messenger system is mediated by G proteins that are associated with the receptor for thyrotropin-releasing hormone. |
Celluar localization | Plasma Membrane; |
UniPort | P34981 |
Data Examples
Our experiment involved the analysis of COS7 cell lysate using a TRHR antibody through western blot. To generate a differentiated content based on the provided information, we can construct a narrative that explores the purpose, methodology, and potential outcomes of the experiment.
The objective of our study was to investigate the expression levels of TRHR (thyrotropin-releasing hormone receptor) in COS7 cells. Western blot analysis, a powerful technique in molecular biology, was employed to detect and quantify the target protein.
In our methodology, COS7 cells were cultured and lysed to obtain the cellular proteins. The lysate was then subjected to electrophoresis to separate the proteins based on their molecular weight. The separated proteins were subsequently transferred onto a membrane and probed with the TRHR antibody. This antibody specifically recognizes and binds to the TRHR protein, allowing for its detection.
The results of this western blot analysis will shed light on the presence and abundance of TRHR in COS7 cells. By comparing the protein bands obtained from the lysate with a molecular weight marker, we can determine the size of the TRHR protein. Additionally, the intensity of the signal generated by the antibody binding will reflect the relative expression level of TRHR in COS7 cells.
Understanding the expression profile of TRHR can have significant implications for elucidating its functional role in cellular processes. It may reveal insights into hormone regulation and the signaling pathways involved. Furthermore, this information can contribute to the development of targeted therapies for disorders related to TRHR dysfunction.
In conclusion, our study employed western blot analysis using a TRHR antibody to investigate the expression of TRHR in COS7 cell lysate. The results obtained from this experiment will provide crucial information, advancing our understanding of TRHR biology and its relevance in cellular processes.
This product is for research use only, not for use in diagnostic prodecures or in human.
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