Rabbit Anti-Caspase 10 Polyclonal Antibody #abs130059

abs130059-50ug

1-2 Weeks

201

abs130059-100ug

1-2 Weeks

301

Description

Casp10 is a crucial protein involved in the mechanism of apoptosis execution mediated by caspases. Its activation cascade is triggered by both Fas and TNFR-1 receptors, which recruit Casp10 in a FADD dependent manner. In addition, it may also participate in the granzyme B apoptotic pathways. Casp10 is known to cleave and activate various caspases such as caspase-3, -4, -6, -7, -8, and -9. Furthermore, it is able to hydrolyze small-molecule substrates like Tyr-Val-Ala-Asp-|-AMC and Asp-Glu-Val-Asp-|-AMC.
Casp10 belongs to the peptidase C14A family and forms a heterotetramer consisting of two antiparallel arranged heterodimers. Each heterodimer is made up of a 23/17 kDa (p23/17) subunit (depending upon splicing events) and a 12 kDa (p12) subunit. Casp10 also has self-associating properties and interacts with FADD and CASP8. It is found in a Fas signaling complex that comprises of FAS, FADD, CASP8, and CASP10.
It is worth noting that human Casp10 has 6 isoforms that are produced through alternative splicing. In summary, Casp10 is a versatile protein that plays a pivotal role in the regulation of apoptotic pathways, and its activity is controlled through interactions with other proteins and its own capacity to self-associate.

Other names

CASP10, also known as ALPS2, Apoptotic protease Mch-4, or FAS-associated death domain protein, is a cysteine peptidase involved in apoptosis. It is encoded by the CASP10 gene and referred to as CASPA_HUMAN. CASP10 is a subunit p12 of Caspase 10, and it is also known as FADD like ICE2 or FLICE2. Additionally, it is identified as ICE-like apoptotic protease 4 or Interleukin 1B converting enzyme 2. CASP10, or MCH 4, plays a crucial role in the regulation of cell death pathways.

Source

Rabbit

Specificity

The endogenous levels of total Caspase 10 are accurately detected by the Caspase 10 antibody. You can rely on this antibody to measure the presence of Caspase 10 in the sample.

Species Reactivity

Human;Mouse

Predictive reaction species

Horse

Application

The recommended dilutions for different applications are as follows:
- For Western blot (WB), the suggested dilution ranges from 1:500 to 1:3000.
- For immunohistochemistry (IHC), the optimal dilution is between 1:50 and 1:200.
- For immunofluorescence/immunocytochemistry (IF/ICC), the appropriate dilution is in the range of 1:100 to 1:500.
- For ELISA using a peptide as the antigen, the recommended dilution ranges from 1:20000 to 1:40000.
Please note that these dilutions are based on the original specifications provided.

Immunogen

A peptide synthesized from Caspase 10 found in humans has been developed. The synthesized product was designed to closely resemble the properties of the original human Caspase 10. Efforts were geared towards ensuring that the properties of the synthetic version of the Caspase 10 were highly similar to the original human Caspase 10. This was achieved by carefully manipulating various parameters such as the molecular structure, composition, and other relevant properties. The aim was to create a product that would be more readily available and easier to handle than the human Caspase 10. This accomplishment has paved the way for further research in the field of synthetic peptides and human Caspase 10.

Properties

Concentration

1mg/ml

Purification

Using SulfoLink™ Coupling Resin, the antiserum was subjected to peptide affinity chromatography to purify it. A process of separating impurities from the antiserum was carried out through this technique. This resulted in a highly purified form of the antiserum, ready for further use.

Clonality

Polyclonal Antibody

Stability & Storage

To ensure optimal preservation, it is recommended to store the item at a temperature of -20 °C for a duration of one year. It is important to avoid subjecting it to multiple freeze/thaw cycles.

Storage buffer

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Target

Background

Involved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates caspase-3, -4, -6, -7, -8, and -9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-|-AMC and Asp-Glu-Val-Asp-|-AMC.

Tissue specificity

Detectable in most tissues. Lowest expression is seen in brain, kidney, prostate, testis and colon.

Posttranslational modification

Cleavage by granzyme B and autocatalytic activity generate the two active subunits.

Celluar localization

Cytosol;Plasma Membrane;

UniPort

Q92851