Rabbit Anti-alpha-SMA Polyclonal Antibody#abs130621

abs130621-50ug

1-2 Weeks

201

abs130621-100ug

1-2 Weeks

301

Description

AAT6, also known as familial thoracic aortic aneurysm, is caused by defects in the ACTA2 gene. This condition is characterized by the permanent enlargement of the thoracic aorta, typically resulting from degenerative changes in the aortic wall. A key feature of AAT6 is the presence of "medial necrosis" or "Erdheim cystic medial necrosis," which refers to the degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance within the aortic wall.

Other names

Actin, also known as alpha actin 2 or sarcomeric actin, is a protein found in smooth muscle tissue, including the aorta. It is encoded by the AAT6 gene and is also referred to as ACTA_HUMAN or ACTA2. Other names for actin include ACTSA and ACTVS.
Actin is involved in a variety of cellular processes, including cell growth inhibition. It is also known as the growth inhibiting gene 46 protein, or GIG46, and the cell growth-inhibiting gene 46 protein.
Alpha cardiac actin and alpha-actin-2 are also alternate names for actin. It plays an important role in muscle contraction and is important for the structure and function of smooth muscle tissue.
In summary, actin is a crucial protein with various functions in smooth muscle tissue. It is expressed in the aortic smooth muscle and is encoded by the AAT6 gene. Actin is critical for muscle contraction and is involved in cellular processes such as cell growth inhibition.

Source

Rabbit

Specificity

Endogenous levels of alpha-SMA can be detected using the alpha-SMA antibody. This antibody is designed to specifically target alpha-SMA molecules within the cell, allowing for accurate and reliable detection of this important protein. By detecting levels of alpha-SMA, researchers can gain important insights into cellular processes such as contraction, migration, and differentiation. Whether in vitro or in vivo, the alpha-SMA antibody is a powerful and widely used tool for investigating cellular behavior at the molecular level.

Predictive reaction species

Chicken;Rabbit;Pig;Dog;Sheep;Horse;Bovine

Application

The dilutions for Western blot (WB) range from 1:500 to 1:1000, for immunohistochemistry (IHC) they range from 1:50 to 1:500, for immunofluorescence (IF) it is 1:200, for immunoprecipitation (IP) it is 1:100, and for ELISA (peptide) it ranges from 1:20000 to 1:40000. These dilutions are used to optimize the detection of specific proteins or peptides in various experimental techniques. It is important to carefully adjust the dilutions to achieve optimal results and ensure the accurate detection of the target molecules.

The SulfoLink™ Coupling Resin was utilized in the purification of the antiserum through peptide affinity chromatography. The resulting product was highly refined, ensuring the removal of all impurities.

Properties

1mg/ml

Purification

The purified rabbit serum was utilized to obtain the antibody through a sequence of chromatography steps. These steps involved the use of phospho- and non-phospho-peptide affinity columns for affinity purification. The resultant antibody is highly pure and similar to the original rabbit serum, making it suitable for downstream applications.

Clonality

Polyclonal Antibody

To maintain optimal conditions, it is advised to store the product at a temperature of -20 °C for a duration of one year. It is important to prevent the occurrence of multiple freeze-thaw cycles.

Storage buffer

This product contains Rabbit IgG in a solution of phosphate buffered saline with a pH of 7.4. The solution also includes 150mM of NaCl and 0.02% of sodium azide. To enhance stability, 50% glycerol is added. It is recommended to store this product at -20 °C. It has a shelf life of 12 months from the date of receipt.

Target

Background

 Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Tissue specificity

Up-regulated in response to enterovirus 71 (EV71) infection.

Celluar localization

Cytoskeleton;Cytosol;Extracellular region or secreted;

P62736