Rabbit Anti-MMP14 Polyclonal Antibody #abs130148

abs130148-50ug

1-2 Weeks

201

abs130148-100ug

1-2 Weeks

301

Description

It appears that MMP14 has a specific role in activating the enzyme progelatinase A. This activation could potentially lead to invasive behaviors of tumor cells, as progelatinase A is activated on the surface of these cancerous cells. MMP14 is classified as a member of the M10A peptidase family. To summarize, MMP14 plays a significant role in promoting tumor invasion by activating progelatinase A.

Other names

Matrix metallopeptidase 14 is also known as membrane type 1 matrix metalloproteinase, membrane type 1 metalloprotease, or MT1-MMP. It is a protease enzyme that is inserted into the cell membrane. With the gene name MMP14, it is involved in extracellular matrix remodeling and is associated with tumor invasion and metastasis. MT1-MMP plays a crucial role in the degradation of various components of the extracellular matrix, such as collagen and proteoglycans. Its activity is tightly regulated to maintain tissue homeostasis and prevent excessive tissue degradation. MT1-MMP is considered a promising target for anti-cancer therapies due to its involvement in tumor progression and metastasis. Further research on MT1-MMP will provide insights into its mechanisms of action and potential therapeutic interventions.

Source

Rabbit

Specificity

The MMP14 antibody is capable of detecting total levels of endogenous MMP14. This means that the antibody can identify and measure the amount of MMP14 that is naturally present in a sample, without introducing any external factors that could alter the results. With the MMP14 antibody, researchers can gain a better understanding of the role and function of MMP14 in various biological processes.

Species Reactivity

Human;Mouse;Rat

Predictive reaction species

Pig;Horse;

Application

For Western blotting, a dilution range of 1:500 to 1:3000 is recommended. When performing immunohistochemistry, a dilution of 1:50 to 1:200 is optimal. For immunofluorescence/immunocytochemistry, a dilution range of 1:100 to 1:500 is recommended. For ELISA using a peptide substrate, a dilution range of 1:20000 to 1:40000 is recommended for optimal results. These dilution ranges are critical to ensure that the antibody performs optimally and provides reliable results for downstream applications.

Immunogen

A synthesized peptide derived from human MMP14.

Properties

Concentration

1mg/ml

Purification

The purification of the antiserum was carried out through peptide affinity chromatography, which involved the use of SulfoLink™ Coupling Resin to separate the desired molecule from other contaminants.

Clonality

Polyclonal Antibody

Stability & Storage

To ensure the longevity of your product, it is recommended to store it at a temperature of -20°C for a year. However, it is crucial to avoid any repeat freeze/thaw cycles to retain its quality.

Storage buffer

This product is comprised of Rabbit IgG in a phosphate buffered saline solution that has been adjusted to a pH of 7.4. The solution also contains 150mM NaCl, 0.02% sodium azide and 50% glycerol. It is recommended to store this product at -20 °C to ensure maximum stability, and it is guaranteed to remain stable for 12 months from the date of receipt.

Target

Background

The protein under discussion appears to have a specific role in activating progelatinase A, which has the potential to trigger tumor cell invasion. This occurs when progelatinase A is activated on the surface of the tumor cells. In addition to this, the protein may play a key role in reorganizing the actin cytoskeleton by cleaving PTK7. Further, it has been shown that this protein can act as a positive regulator of cell growth and migration by activating MMP15. It is also believed to be involved in the formation of fibrovascular tissues, working in tandem with pro-MMP2. Overall, the protein has complex functions, with implications for tumor progression and cell migration.

Tissue specificity

Expressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors.

Posttranslational modification

The precursor is cleaved by a furin endopeptidase.Tyrosine phosphorylated by PKDCC/VLK.

Celluar localization

Cytoskeleton; Cytosol; Endosome; Extracellular region or secreted; Golgi apparatus; Nucleus; Plasma Membrane;

UniPort

P50281