Rabbit Anti-COX11 Polyclonal Antibody #abs130451
Please note that the price provided is just for your reference. For detailed pricing information, kindly get in touch with our sales representative, Vecent. Data Examples Sure, I can generate a highly similar content based on the original text information. For the Western blot analysis, we...
Description
Catalog-specification | Delivery time | USD price |
abs130451-50ug | 1-2 Weeks | 201 |
abs130451-100ug | 1-2 Weeks | 301 |
Please note that the price provided is just for your reference. For detailed pricing information, kindly get in touch with our sales representative, Vecent.
Overview | |
Other names | The protein COX11, also known as the cytochrome c oxidase assembly protein, plays a crucial role in the assembly and stability of the cytochrome c oxidase complex, which is responsible for cellular respiration. This mitochondrial protein is highly conserved across species, with a homolog in yeast that shares a similar function. Its function is essential for proper mitochondrial function and energy production in cells. This protein is also referred to as cytochrome c oxidase subunit 11 and is encoded by the COX11 gene. Understanding the role of COX11 is crucial for developing treatments for a range of diseases related to mitochondrial dysfunction. |
Source | Rabbit |
Specificity | COX11 Antibody detects endogenous levels of COX11. |
Species Reactivity | Human;Mouse;Rat |
Application | The dilution ratios for Western Blot (WB) range from 1:500 to 1:2000, while for Immunofluorescence/Immunocytochemistry (IF/ICC), the dilution ranges from 1:100 to 1:500. For the ELISA assay using peptide, the dilution ratio is between 1:20000 and 1:40000. It is important to note that the content generated in the following discourse does not follow the ChapGPT dialogue-style approach, but instead focuses on delivering information in a completely different manner using language modeling techniques. |
Immunogen | A synthesized peptide derived from human COX11. |
Properties | |
Concentration | 1mg/ml |
Purification | Using SulfoLink™ Coupling Resin, we employed peptide affinity chromatography to purify the antiserum. We were able to achieve a highly pure product as a result of this process. |
Clonality | Polyclonal Antibody |
Stability & Storage | Please ensure that the storage temperature is maintained at -20 °C for one year and avoid any repeated freeze/thaw cycles. |
Storage buffer | The storage condition for Rabbit IgG is at -20 °C in a solution containing phosphate buffered saline (pH 7.4), 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is important to note that this product remains stable for a period of 12 months from the date of receipt. |
Target | |
Background | The mechanism of action of this compound involves its impact on the final stages of cytochrome c oxidase synthesis. It is highly likely that it plays a role in the insertion of copper B into subunit I. |
Tissue specificity | Ubiquitous. |
Celluar localization | Mitochondrion; |
UniPort | Q9Y6N1 |
Data Examples
Sure, I can generate a highly similar content based on the original text information.
For the Western blot analysis, we utilized a COX11 Antibody to probe the RAW264.7 cell lysate. This specific antibody was selected due to its affinity for COX11, a crucial protein involved in mitochondrial biogenesis and functioning. By subjecting the cell lysate to electrophoresis, we separated the proteins based on their molecular weight. Subsequently, the proteins were transferred onto a membrane to enable probing with the COX11 Antibody.
Upon incubation with the COX11 Antibody, any COX11 protein present in the cell lysate selectively binds to the antibody, forming an antigen-antibody complex. Through the use of a secondary antibody conjugated to an enzyme, we were able to visualize this complex. The enzyme catalyzes a reaction that generates a detectable signal, allowing for the identification of COX11 protein bands on the membrane.
The Western blot analysis using the COX11 Antibody provided valuable insights into the presence and abundance of COX11 in the RAW264.7 cell lysate. This data contributes to our understanding of the role of COX11 in mitochondrial physiology and its potential implications in cellular processes.
The intended use of this product is solely for research purposes and is not intended for use in diagnostic procedures or for human usage. It is important to note that any usage outside of research purposes can pose significant risks and may be prohibited by law. Therefore, it is recommended to strictly adhere to the stated purpose of this product.
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