Rabbit Anti-FAP-1 Polyclonal Antibody #abs130547

abs130547-50ug

1-2 Weeks

201

abs130547-100ug

1-2 Weeks

301

Description

PTPN13 is a member of the protein-tyrosine phosphatase family and belongs to the non-receptor class subfamily. It plays a crucial role in negatively regulating FAS-induced apoptosis and the pro-apoptotic signaling mediated by NGFR. PTPN13 interacts with TRIP6 and TNFRSF6 (Fas receptor) through its second PDZ domain, while its third PDZ domain interacts with the C-terminal SVP motif of NGFR. This intricate network of interactions highlights the importance of PTPN13 in regulating apoptosis and signaling pathways, emphasizing its relevance in cellular homeostasis and disease progression.

Other names

The 170 kDa melanoma membrane-bound gelatinase, also known as DPPIV or Seprase, is an integral membrane serine protease that plays a crucial role in the progression of cancer. FAP (Fibroblast Activation Protein) alpha is another name for Seprase, and it is an enzyme that cleaves proteins. Seprase has been identified in various tumors, including melanoma, breast cancer, lung cancer, and pancreatic cancer. It is also present in the stroma of solid tumors and is thought to be involved in tumor growth and metastasis. Despite being a potential therapeutic target, Seprase's precise mechanism of action remains unclear.

Source

Rabbit

Specificity

FAP-1 Antibody detects endogenous levels of FAP-1

Species Reactivity

Human;Mouse;Rat

Application

The dilution ratios for WB range from 1:500 to 1:2000, while for IHC they range from 1:50 to 1:200. When conducting ELISA with a peptide, the recommended dilution range is 1:20000 to 1:40000. It is important to ensure that the generated content is not derived from the original text but instead presents a distinct way of conveying information.

Immunogen

A synthesized peptide derived from human FAP-1

Properties

Concentration

1mg/ml

Purification

Immunogen affinity purified

Clonality

Polyclonal Antibody

Stability & Storage

Make sure to store the product at a temperature of -20 °C for a duration of one year. It is important to avoid subjecting the product to repeated freeze/thaw cycles during this period.

Storage buffer

This Rabbit IgG solution is formulated with phosphate buffered saline, pH 7.4, 150mM NaCl, and 0.02% sodium azide, along with 50% glycerol. It should be stored at -20°C for optimal stability, with a shelf life of up to 12 months from receipt date. For best results, please handle with care and follow all recommended protocols.

Target

Background

This glycoprotein serine protease, located on the cell surface, is involved in various cellular processes such as tissue remodeling, fibrosis, wound healing, inflammation, and tumor growth. It plays a crucial role in extracellular matrix degradation. Both the soluble and plasma membrane forms of this protease exhibit endopeptidase activity, specifically targeting amino acid sequences with an Ala/Ser-Gly-Pro-Ser/Asn/Ala consensus. It can degrade proteins like alpha-2-antiplasmin and SPRY2, as well as gelatin and heat-denatured type I collagen. However, it does not act on native collagen type I and IV, vibronectin, tenascin, laminin, fibronectin, fibrin, or casein. Interestingly, it also possesses dipeptidyl peptidase activity, breaking down dipeptides with a proline residue at the penultimate position, with a preference for certain amino acid combinations. Neuropeptide hormones like neuropeptide Y (NPY), peptide YY (PYY), substance P (TAC1), and brain natriuretic peptide 32 (NPPB) serve as natural substrates for its dipeptidyl peptidase activity. In association with DPP4, PLAUR, or integrins, the plasma membrane form of this protease contributes to pericellular proteolysis of the extracellular matrix, thereby promoting cell adhesion, migration, and invasion. It is also involved in tissue remodeling during development and wound healing. In malignant melanomas, it enhances cell invasiveness through the extracellular matrix. Furthermore, it promotes tumor growth progression by increasing angiogenesis, collagen fiber degradation, apoptosis, and inhibiting the immune system's antitumor response. However, it acts as a tumor suppressor in melanocytic cells, regulating cell proliferation and survival independently of its serine protease activity.

Tissue specificity

Expressed in adipose tissue. Expressed in the dermal fibroblasts in the fetal skin. Expressed in the granulation tissue of healing wounds and on reactive stromal fibroblast in epithelial cancers. Expressed in activated fibroblast-like synoviocytes from inflamed synovial tissues. Expressed in activated hepatic stellate cells (HSC) and myofibroblasts from cirrhotic liver, but not detected in normal liver. Expressed in glioma cells (at protein level). Expressed in glioblastomas and glioma cells. Isoform 1 and isoform 2 are expressed in melanoma, carcinoma and fibroblast cell lines.

Posttranslational modification

N-glycosylated.The N-terminus may be blocked.

Celluar localization

Extracellular region or secreted;Plasma Membrane;

UniPort

Q12884