Rabbit Anti-Fibronectin1 Polyclonal Antibody #abs130546

abs130546-50ug

1-2 Weeks

201

abs130546-100ug

1-2 Weeks

301

Description

Fibronectins are adhesive molecules that bind to various molecules such as collagen, fibrin, heparin, DNA, and actin. They play critical roles in cell adhesion, cell motility, wound healing, opsonization, and maintaining cell shape. These proteins exist mainly as heterodimers or multimers of alternatively spliced variants connected by two disulfide bonds at the carboxyl ends, with homodimers seen less frequently. Fibronectins interact with a range of proteins, including FBLN1, AMBP, TNR, LGALS3BP, and COL13A1, while they interact with FBLN7 and COMP. Overall, fibronectins are versatile molecules that play crucial roles in maintaining cells' structural integrity and facilitating cell processes such as migration and wound closure.

Other names

Fibronectin 1, also known as FN or ED B, is a protein that contains cold-insoluble globulin domains and plays a role in cell adhesion and migration. It is encoded by the FN1 gene and is found in various tissues throughout the body. FN is involved in wound healing, embryonic development, and maintenance of normal tissue architecture. It can also bind to other extracellular matrix proteins and growth factors, contributing to cell signaling and tissue remodeling. MSF, GFND, and LETS are other names for FN. Overall, FN is a multifaceted protein with important biological functions.

Source

Rabbit

Specificity

The Fibronectin 1 Antibody is capable of identifying the levels of endogenous Fibronectin 1 in a sample. This antibody targets Fibronectin 1 and can detect its presence in a reliable and efficient manner. Whether you're studying the expression patterns of Fibronectin 1 or performing quantitative analysis, this antibody can provide accurate and consistent results. With its ability to recognize Fibronectin 1 at endogenous levels, this antibody is an invaluable tool for any researcher interested in studying this important protein.

Species Reactivity

Human;Mouse;Rat

Application

The appropriate dilution range for Western blot (WB) is 1:500-1:2000, while for immunohistochemistry (IHC) it is 1:50-1:200. As for immunofluorescence/immunocytochemistry (IF/ICC), the recommended dilution range is 1:100-1:500. For ELISA using peptide as the antigen, the suitable dilution range is 1:20000-1:40000.

Immunogen

A synthesized compound has been developed utilizing human Fibronectin 1 as its source material. The peptide is derived from this protein and has been designed to closely mimic its structure and function in the body. With this innovative creation, researchers hope to gain a greater understanding of the underlying mechanisms involved in Fibronectin 1, as well as to explore potential therapeutic applications for this compound. The synthesized peptide represents a promising avenue for future research into the various roles played by Fibronectin 1 in supporting a healthy body.

Properties

Concentration

1mg/ml

Purification

Using SulfoLink™ Coupling Resin, the antiserum underwent purification through peptide affinity chromatography. This process allowed for the removal of impurities and isolation of a highly purified product.

Clonality

Polyclonal Antibody

Stability & Storage

To maintain the longevity of the product, it is advised to store it at a temperature of -20 °C for a period of one year. It is recommended to avoid subjecting the product to repeated cycles of freezing and thawing.

Storage buffer

The storage condition for Rabbit IgG is in phosphate buffered saline at pH 7.4, with a concentration of 150mM NaCl and 0.02% sodium azide. Additionally, it should be stored in a solution containing 50% glycerol at -20 °C. This storage condition ensures its stability for a period of 12 months from the date of receipt.

Target

Background

Fibronectins play a key role in binding various compounds, such as collagen, fibrin, heparin, DNA, and actin, to cell surfaces. These proteins are critical for cell adhesion, cell motility, and maintenance of cell shape, as well as for important biological processes like opsonization and wound healing. In addition, they are crucial for osteoblast compaction, facilitating the mineralization process by contributing to the fibronectin fibrillogenesis cell-mediated matrix assembly. Moreover, fibronectins are involved in the regulation of collagen deposition by osteoblasts, highlighting their importance in the maintenance of bone health and function.

Tissue specificity

Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.

Posttranslational modification

Sulfated.It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).Phosphorylated by FAM20C in the extracellular medium.Proteolytic processing produces the C-terminal NC1 peptide, anastellin.Some lysine residues are oxidized to allysine by LOXL3, promoting fibronectin activation and matrix formation.

Celluar localization

Endoplasmic reticulum;Extracellular region or secreted;Plasma Membrane;

UniPort

P02751