Rabbit Anti-CLIM1 Polyclonal Antibody #abs130239

abs130239-50ug

1-2 Weeks

201

abs130239-100ug

1-2 Weeks

301

Description

CLIM1 is an adaptor protein that is responsible for linking other proteins to the cytoskeleton. It mainly interacts with alpha-actinins 1, 2, and 4, and recruits the Clik1 kinase to actin stress fibers in nonmuscle cells. This protein is highly expressed in the heart and skeletal muscles, and moderately expressed in the spleen, small intestine, colon, placenta, and lungs. It has a lower level of expression in the liver, thymus, kidney, prostate, and pancreas. Interestingly, it is not found in the brain, testis, ovary, and peripheral blood leukocytes. This suggests that CLIM1 plays an essential role in maintaining the cytoskeletal structure in specialized cell types.

Other names

PDLIM1_HUMAN is a protein commonly known as C-terminal LIM domain protein 1 or CLP36. It is also referred to as carboxy terminal LIM domain protein 1. This protein is encoded by the CLP-36 gene and is found in humans. It is part of the LIM domain protein family and is involved in various cellular processes.
Another name for PDLIM1_HUMAN is Elfin, which is its alternative name. It is also known as Enigma homolog. The protein hCLIM1 or CLIM1 is another name used for PDLIM1_HUMAN. This protein contains both PDZ and LIM domains. The PDZ and LIM domain 1 protein is the full name of PDLIM1_HUMAN. It is often abbreviated as CLIM1.
PDLIM1_HUMAN is important for the regulation of protein-protein interactions in cells. It plays a role in the assembly of protein complexes and contributes to cellular signaling pathways. The precise function of PDLIM1_HUMAN is not fully understood, but it is known to be involved in cell adhesion, cytoskeletal organization, and gene expression regulation.
In summary, PDLIM1_HUMAN is a versatile protein with multiple names such as C-terminal LIM domain protein 1, Elfin, Enigma homolog, and hCLIM1. It contains both PDZ and LIM domains and is involved in various cellular processes. Further research is needed to fully understand its function and significance in cellular regulation.

Source

Rabbit

Specificity

The total CLIM1 in the body can be detected by the CLIM1 antibody at the endogenous levels. To create a similar content, one can rephrase the original information in different words while preserving the meaning and message of the text.

Species Reactivity

Human;Mouse;Rat

Application

The recommended dilutions for different applications are as follows: Western blotting (WB) can be performed with dilutions ranging from 1:500 to 1:3000. For immunohistochemistry (IHC), the suitable dilutions range from 1:50 to 1:200. If you are planning on conducting immunofluorescence (IF) or immunocytochemistry (ICC), then the recommended dilutions are 1:100 to 1:500. Lastly, for ELISA assays using peptide antigens, the appropriate dilutions are recommended to be between 1:20000 and 1:40000.

Immunogen

A synthesized peptide derived from human CLIM1

Properties

Concentration

1mg/ml

Purification

Affinity-chromatography

Clonality

Polyclonal Antibody

Stability & Storage

To preserve the product's quality, it is recommended to store it at a temperature of -20 °C for a period of one year. It is important to avoid subjecting the product to repeated freeze/thaw cycles as this may affect its integrity.

Storage buffer

The recommended storage conditions for Rabbit IgG are as follows: it should be stored at a temperature of -20 °C in a solution containing phosphate buffered saline with a pH of 7.4. The solution should also contain 150mM NaCl, 0.02% sodium azide, and 50% glycerol. When stored under these conditions, the Rabbit IgG is stable for a period of 12 months from the date of receipt. It is important to ensure that the storage temperature is maintained at -20 °C to preserve the integrity and functionality of the Rabbit IgG.

Target

Background

A protein found in the cytoskeleton, it is believed to function as an adaptor that can attract other proteins such as kinases to this cellular structure.

Tissue specificity

Strongly expressed in the heart and skeletal muscle, moderately expressed in the spleen, small intestine, colon, placenta, and lung. A lower level expression is seen in liver, thymus, kidney, prostate and pancreas and is not found in the brain, testis, ovary, and peripheral blood leukocytes.

Celluar localization

Cytoskeleton;

UniPort

O00151