Home - Products - Antibodies - Primary Antibodies - Polyclonal Antibody - Details

Rabbit Anti-HMGCR Polyclonal Antibody#abs136038

Please note that the price provided is only for your reference. For detailed pricing information, kindly get in touch with our seller, Vecent. Western blot analysis of extracts of hela cell lines, using HMGCR antibody. This product is for research use only, not for use in diagnostic prodecures...

Send Inquiry

Description

Catalog-specification	Delivery time	USD price
abs136038-100ug	1-2 Weeks	301.0
abs136038-50ug	1-2 Weeks	201.0

Please note that the price provided is only for your reference. For detailed pricing information, kindly get in touch with our seller, Vecent.

Overview
catalog	abs136038
Description	HMG-CoA reductase is the key enzyme responsible for the biosynthesis of cholesterol and is regulated through negative feedback by sterol and non-sterol metabolites derived from mevalonate, which is the product of the reaction catalyzed by the reductase. In mammalian cells, cholesterol inhibits this enzyme through the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase promote the expression of LDL receptors in the liver, increasing the catabolism of plasma LDL and decreasing the plasma concentration of cholesterol, a critical determinant of atherosclerosis. Multiple isoforms of this gene have been identified via alternatively spliced transcript variants. [provided by RefSeq, Aug 2008].
Other names	The enzyme known as 3-hydroxy-3-methylglutaryl CoA reductase is also referred to as HMG CoA reductase, HMG CoAR, Hmgcr, and 3H3M. It plays an important role in the biosynthesis of cholesterol by reducing 3-hydroxy-3-methylglutaryl CoA to mevalonate. The enzyme requires NADPH as a cofactor to carry out this reaction. It is a target for drugs such as statins, which are used to lower cholesterol levels in the body. In humans, the HMG-CoA reductase gene is located on chromosome 5. In addition, this enzyme is also known as hydroxymethylglutaryl CoA reductase or 3-hydroxy-3-methylglutaryl-coenzyme A reductase. LDLCQ3 and MGC103269 are alternate names for this gene.
Source	Rabbit
Specificity	The detection capability of HMGCR Antibody is specific to the endogenous levels of total HMGCR. To provide similar content, the antibody is designed to accurately identify and measure the amount of total HMGCR present in a sample. By targeting the endogenous HMGCR protein, the antibody ensures reliable and accurate detection of its levels. This capability is valuable for various research applications where understanding the total amount of HMGCR is crucial. To summarize, the HMGCR Antibody offers a highly effective means of detecting and quantifying endogenous total HMGCR levels.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Bovine;Horse;
Antigen	HMGCR
Application	The dilutions for Western Blot (WB) range from 1:500 to 1:2000, for Immunohistochemistry (IHC) they range from 1:50 to 1:200, and for ELISA (peptide) they range from 1:20000 to 1:40000. Please note that the provided dilution ratios are based on the original text information.
Immunogen	A synthesized peptide derived from human HMGCR.
MW	95kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Transmembrane glycoprotein that is the rate-limiting enzyme in cholesterol biosynthesis as well as in the biosynthesis of nonsterol isoprenoids that are essential for normal cell function including ubiquinone and geranylgeranyl proteins.
Posttranslational modification	N-glycosylated. Deglycosylated by NGLY1 on release from the endoplasmic reticulum (ER) in a sterol-mediated manner.Undergoes sterol-mediated ubiquitination and ER-association degradation (ERAD). Accumulation of sterols in the endoplasmic reticulum (ER) membrane, triggers binding of the reductase to the ER membrane protein INSIG1. This INSIG1 binding leads to the recruitment of the ubiquitin ligase, AMFR/gp78, initiating ubiquitination of the reductase. The ubiquitinated reductase is then extracted from the ER membrane and delivered to cytosolic 26S proteosomes by a mechanism probably mediated by the ATPase Valosin-containing protein VCP/p97. Lys-248 is the main site of ubiquitination. Ubiquitination is enhanced by the presence of a geranylgeranylated protein.
Celluar localization	Endoplasmic reticulum;Peroxisome;
UniPort	P04035