Rabbit Anti-Galectin3 Polyclonal Antibody #abs130101

abs130101-50ug

1-2 Weeks

201

abs130101-100ug

1-2 Weeks

301

Description

Galectin-3 is a type of lectin that works specifically with galactose and has a high affinity to IgE. This protein is expressed in high levels in the colonic epithelium, where it performs various functions. It is also present in activated macrophages, playing a crucial role in immunity and inflammation. With its unique binding abilities, galectin-3 is a potential target for diagnostic and therapeutic purposes.

Other names

Galectin 3, also known as carbohydrate binding protein 35 or CBP 35, is a lectin that binds to carbohydrates. It has a molecular weight of 35 kDa and is involved in various biological processes, including cell adhesion, apoptosis, and immune response. Galectin 3 is also known as LGALS3 or MAC2 antigen and is expressed on the surface of various cells, including macrophages and epithelial cells. It is a galactose-specific lectin and is involved in the recognition and binding of beta-galactoside-containing glycoconjugates. The protein also has a laminin-binding domain, which allows it to interact with laminin and modulate cellular responses. It has been suggested that galectin 3 may play a role in cancer progression and metastasis by activating various signaling pathways. Overall, galectin 3 is a multifunctional protein that plays a critical role in various physiological and pathological processes.

Source

Rabbit

Specificity

The Galectin 3 antibody is capable of identifying the complete amount of Galectin 3 present within the body. This indicates that the antibody is capable of detecting the endogenous levels of total Galectin 3.

Species Reactivity

Human;Mouse;Rat

Application

Western blot (WB) typically requires a dilution range of 1:500~1:3000. Immunohistochemistry (IHC) is usually performed at dilutions of 1:50~1:200. For immunofluorescence/immunocytochemistry (IF/ICC), the recommended dilutions are 1:100~1:500. In the case of ELISA using peptide antigens, a dilution range of 1:20000-1:40000 is commonly used. These dilution ranges are important for achieving optimal results in each assay.

Immunogen

A peptide synthesized from the human Galectin 3 has been developed as a potential therapeutic agent. This peptide is a mimic of the natural glycan-binding domain found in Galectin 3 and has been shown to have promising activity in preclinical studies. Galectin 3 is a multifunctional protein that plays a role in a variety of biological processes, including cell adhesion, apoptosis, and immunity. Its expression has been linked to several diseases, including cancer and inflammation, making it an attractive target for therapeutic intervention. The synthesized peptide offers a novel approach to modulating Galectin 3 activity and may have potential clinical applications in the future.

Properties

Concentration

1mg/ml

Purification

Using SulfoLink™ Coupling Resin, the purification of the antiserum was achieved through peptide affinity chromatography.

Clonality

Polyclonal Antibody

Stability & Storage

20，/。

Storage buffer

This product contains rabbit IgG in a solution of phosphate buffered saline, with a pH of 7.4 and a concentration of 150 millimolar sodium chloride. In addition, the solution contains 0.02% sodium azide and 50% glycerol. It is recommended to store this product at a temperature of -20 degrees Celsius. This product is stable for a period of twelve months from the date of receipt. Please note that the above information is intended to provide an accurate summary of the product specifications.

Target

Background

During early embryogenesis, a galactose-specific lectin plays a crucial role by binding to IgE and mediating the stimulation of endothelial cells migration through the alpha-3, beta-1 integrin. Additionally, this lectin, along with DMBT1, is essential for the terminal differentiation of columnar epithelial cells. In the nucleus, it acts as a pre-mRNA splicing factor. This versatile protein also participates in acute inflammatory responses, contributing to activities such as neutrophil activation and adhesion, chemoattraction of monocytes and macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. The wide range of functions underscores the significance of this galactose-specific lectin in various biological processes.

Tissue specificity

A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages. Expressed in fetal membranes.

Celluar localization

Extracellular region or secreted;Mitochondrion;Nucleus;Plasma Membrane;

UniPort

P17931