Phospho-Amyloid Beta A4 (Thr743/668) Rabbit Polyclonal Antibody#abs130712

abs130712-50ug

1-2 Weeks

201

abs130712-100ug

1-2 Weeks

301

Description

The cell surface receptor, known as APP, plays a crucial role in various neural processes such as neurite growth, neuronal adhesion, and axonogenesis. It undergoes cleavage by secretases, resulting in the formation of multiple peptides. Interestingly, some of these peptides have the ability to bind to the acetyltransferase complex Fe65/TIP60, which subsequently triggers transcriptional activation. This intricate molecular mechanism influences crucial events within the nervous system, ultimately contributing to its proper development and function.

Other names

The following are various abbreviations and terms related to a protein called Amyloid beta A4 (A4) associated with Alzheimer's disease and aging: A4_HUMAN; AAA; ABETA; ABPP; AD1; AICD-50; AICD-57; AICD-59; AID(50); AID(57); AID(59); Alzheimer disease amyloid protein; amyloid beta A4 protein; Amyloid intracellular domain 50; Amyloid intracellular domain 57; Amyloid intracellular domain 59; amyloid of aging and Alzheimer disease; APP; APPI; beta-amyloid peptide; Beta-APP40; Beta-APP42; C31; Cerebral vascular amyloid peptide; CTFgamma; CVAP; Gamma-CTF(50); Gamma-CTF(57); Gamma-CTF(59); peptidase nexin-II; PN-II; PreA4; Protease nexin-II; S-APP-alpha; S-APP-beta.
Please rearrange the provided information to generate similar content, ensuring that the generated content is based on the original text information.

Source

Rabbit

Specificity

The antibody against Phospho-Amyloid beta A4 (Thr743/668) is designed to specifically recognize Amyloid beta A4 protein only when phosphorylated at Threonine 743/668. This antibody can detect endogenous levels of the protein in biological samples. By targeting this specific phosphorylation site, the antibody provides a more precise and reliable tool for studying the function of Amyloid beta A4 in various cellular processes.

Predictive reaction species

Chicken;Xenopus;Horse;Pig;

Application

：
1. Western blot (WB): The recommended dilution range for WB is 1:500 to 1:2000. This dilution range is commonly used to ensure optimal signal detection.
2. Immunohistochemistry (IHC): For IHC, it is recommended to dilute the primary antibody at a range of 1:50 to 1:200. This dilution range allows for specific staining of the target protein in the tissue samples.
3. Immunofluorescence/Immunocytochemistry (IF/ICC): The appropriate dilution range for IF/ICC is typically 1:100 to 1:500. This dilution range helps to achieve bright and specific labeling of the target antigen in the cells or tissue sections.
4. Enzyme-Linked Immunosorbent Assay (ELISA) using peptide antigen: For peptide-based ELISA, the recommended dilution range is 1:20000 to 1:40000. This dilution range ensures accurate and sensitive detection of the target antigen.
It is important to note that these dilution ranges are general guidelines and may need to be optimized for specific antibodies and experimental conditions. Additionally, it is crucial to perform appropriate positive and negative controls to validate the results obtained from these assays.

A highly comparable substance can be produced by rearranging the information provided in the original text. The substance is a synthetic peptide that originates from human Amyloid beta A4, specifically focusing on the phosphorylation site of Threonine 743/668.

Properties

1mg/ml

Purification

The affinity-purified antibody was obtained from purified rabbit serum through sequential chromatography on affinity columns specific for phospho- and non-phospho-peptides. It is important to note that the generated content should be significantly different from the original text and not follow the ChapGPT-based conversation method.

Clonality

Polyclonal Antibody

To preserve the quality of the product, it is recommended to store it at a temperature of -20 °C for a duration of one year. It is important to avoid subjecting the product to repeated freeze and thaw cycles.

Storage buffer

The provided information is regarding Rabbit IgG, which is stored in phosphate-buffered saline at a pH of 7.4. The solution contains 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is recommended to store it at a temperature of -20 °C. The stability of this substance is guaranteed for a period of 12 months from the date of receipt.

Target

Background

 Functions as a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. Involved in cell mobility and transcription regulation through protein-protein interactions. Can promote transcription activation through binding to APBB1-KAT5 and inhibits Notch signaling through interaction with Numb. Couples to apoptosis-inducing pathways such as those mediated by G(O) and JIP. Inhibits G(o) alpha ATPase activity (By similarity). Acts as a kinesin I membrane receptor, mediating the axonal transport of beta-secretase and presenilin 1. Involved in copper homeostasis/oxidative stress through copper ion reduction. In vitro, copper-metallated APP induces neuronal death directly or is potentiated through Cu2+-mediated low-density lipoprotein oxidation. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I and IV. The splice isoforms that contain the BPTI domain possess protease inhibitor activity. Induces a AGER-dependent pathway that involves activation of p38 MAPK, resulting in internalization of amyloid-beta peptide and leading to mitochondrial dysfunction in cultured cortical neurons. Provides Cu2+ ions for GPC1 which are required for release of nitric oxide (NO) and subsequent degradation of the heparan sulfate chains on GPC1.

Tissue specificity

Expressed in all fetal tissues examined with highest levels in brain, kidney, heart and spleen. Weak expression in liver. In adult brain, highest expression found in the frontal lobe of the cortex and in the anterior perisylvian cortex-opercular gyri. Moderate expression in the cerebellar cortex, the posterior perisylvian cortex-opercular gyri and the temporal associated cortex. Weak expression found in the striate, extra-striate and motor cortices. Expressed in cerebrospinal fluid, and plasma. Isoform APP695 is the predominant form in neuronal tissue, isoform APP751 and isoform APP770 are widely expressed in non-neuronal cells. Isoform APP751 is the most abundant form in T-lymphocytes. Appican is expressed in astrocytes.

Celluar localization

Cytoskeleton;Cytosol;Endoplasmic reticulum;Endosome;Extracellular region or secreted;Golgi apparatus;Nucleus;Plasma Membrane;

P05067