Phospho-Integrin Beta3 (Tyr773) Rabbit Polyclonal Antibody#abs130713

abs130713-50ug

1-2 Weeks

201

abs130713-100ug

1-2 Weeks

301

Description

Alpha-V/beta-3 integrin, known as ITGB3, functions as a receptor for various proteins including cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin, and von Willebrand factor. This receptor plays a significant role in mediating cell adhesion and signaling processes. By interacting with these diverse proteins, ITGB3 contributes to numerous physiological and pathological processes in the body. Nonetheless, it is important to note that further research is necessary to fully elucidate the intricate mechanisms underlying the interactions between ITGB3 and its ligands.

Other names

The platelet glycoprotein IIIa antigen, also known as CD61 or integrin beta-3, is an important protein involved in platelet function. It is encoded by the ITGB3 gene and is also commonly referred to as GPIIIa or GP3A. CD61 plays a significant role in blood clotting and is responsible for the formation of fibrinogen receptors in the platelets. Additionally, mutations in this gene have been associated with disorders such as neonatal alloimmune thrombocytopenia (NAIT) as well as susceptibility to cardiovascular disease. It is important to closely study and understand the functions of CD61 in order to find effective treatments for these disorders and improve overall human health.

Source

Rabbit

Specificity

The Phospho-Integrin beta3 (Tyr773) Antibody is designed to specifically recognize and bind to Integrin beta3 protein that has been phosphorylated at Tyrosine 773. This antibody is capable of detecting endogenous levels of phosphorylated Integrin beta3, indicating its function in cellular signaling pathways. By detecting the phosphorylation of Integrin beta3 at Tyr773, this antibody provides valuable insight into the activation and regulation of Integrin beta3-mediated processes.

Predictive reaction species

Rabbit;Bovine;Dog;Horse;

Application

The recommended dilution range for the antibody in different applications is as follows: For Western Blotting (WB) the suggested dilution range is between 1:500-1:2000. For Immunohistochemistry (IHC) the dilution range should be within 1:50-1:200. When it comes to Immunofluorescence (IF) or Immunocytochemistry (ICC), you should dilute the antibody within the range of 1:100-1:500. If you intend to use the antibody in an ELISA (peptide) protocol, then you must dilute it within the recommended range of 1:20000-1:40000. It is important to stick to these recommended dilution ranges for optimal results.

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Properties

1mg/ml

Purification

Purified rabbit serum was used to obtain the antibody through affinity purification, which involved sequential chromatography on phospho- and non-phospho-peptide affinity columns. An advanced purification technique was employed to isolate the desired antibody from the serum. This involved utilizing specialized affinity columns that selectively bind phospho- and non-phospho-peptides, leading to the purification of the antibody.

Clonality

Polyclonal Antibody

To preserve the quality of the product, it is recommended to store it at -20 °C for a duration of one year. It is advisable to avoid subjecting the product to repeated freeze/thaw cycles, as this may affect its effectiveness. Therefore, it is important to handle the product with care to ensure optimal performance.

Storage buffer

This rabbit IgG comes in a phosphate buffered saline solution with a pH of 7.4, 150mM sodium chloride, 0.02% sodium azide, and 50% glycerol. It should be stored in a -20 °C freezer and is stable for 12 months from the date of receipt. This product is highly recommended for anyone in need of a reliable source of rabbit IgG.

Target

Background

 Integrin alpha-V/beta-3 (ITGAV:ITGB3) is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 (ITGA2B:ITGB3) is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. Fibrinogen binding enhances SELP expression in activated platelets (By similarity). ITGAV:ITGB3 binds to fractalkine (CX3CL1) and acts as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed:23125415, PubMed:24789099). ITGAV:ITGB3 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGAV:ITGB3 binds to FGF1 and this binding is essential for FGF1 signaling (PubMed:18441324). ITGAV:ITGB3 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:19578119). ITGAV:ITGB3 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:18635536, PubMed:25398877). ITGAV:ITGB3 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1 (PubMed:12807887).

Tissue specificity

Isoform beta-3A and isoform beta-3C are widely expressed. Isoform beta-3A is specifically expressed in osteoblast cells; isoform beta-3C is specifically expressed in prostate and testis.

Celluar localization

Extracellular region or secreted;Nucleus;Plasma Membrane;

P05106