Rabbit Anti-5-HT-1A Polyclonal Antibody #abs130368

abs130368-50ug

1-2 Weeks

201

abs130368-100ug

1-2 Weeks

301

Description

The inhibitory activity of adenylate cyclase is modulated by a specific receptor for 5-hydroxytryptamine (serotonin). This receptor is part of a larger group of receptors that interact with serotonin, a biogenic hormone with multifunctional roles as a neurotransmitter, a hormone, and a mitogen. G proteins are responsible for mediating the activity of this receptor, ultimately leading to the inhibition of adenylate cyclase.

Other names

The 5 HT receptor 1A, also known as the 5 hydroxytryptamine (serotonin) receptor 1A, is a G protein-coupled receptor that plays a vital role in regulating serotonin levels in the brain. This receptor is responsible for mediating the effects of serotonin on mood, appetite, and sleep, among other physiological functions. It is encoded by the Htr1a gene and can be found in various regions of the brain, including the hippocampus, cortex, and amygdala. Other names for this receptor include 5-HT-1A, 5HT1A, and Serotonin 1A receptor. Dysfunction of this receptor has been implicated in various neuropsychiatric disorders such as depression, anxiety, and schizophrenia. Understanding the mechanisms of this receptor and its interactions with other proteins may lead to the development of more effective treatments for these disorders.

Source

Rabbit

Specificity

The 5-HT-1A Antibody effectively detects the naturally occurring levels of 5-HT-1A in the body's system.

Species Reactivity

Human;Mouse;Rat

Application

，。：
IF/ICC 1:100-1:500，ELISA(peptide) 1:20000-1:40000，WB 1:500-1:2000。，。/，1:1001:500。（ELISA），1:200001:40000。Western，1:5001:2000。，。

Immunogen

A synthesized peptide derived from human 5-HT-1A.

Properties

Concentration

1mg/ml

Purification

Using SulfoLink™ Coupling Resin, the antiserum was subjected to peptide affinity chromatography for purification. It undergoes a process where the resin binds with the target protein or peptide, removing any contaminating materials in the process. The result is a highly purified antiserum that can be used for various applications.

Clonality

Polyclonal Antibody

Stability & Storage

It is recommended to store the product at a temperature of -20 °C for a duration of one year. To maintain the quality of the product, it is important to avoid subjecting it to repeated freeze/thaw cycles.

Storage buffer

This solution contains Rabbit IgG in a phosphate buffered saline solution with a pH of 7.4. It also contains 150mM of NaCl, 0.02% of sodium azide, and 50% glycerol. It is recommended to store this solution at a temperature of -20 °C, as it will remain stable for up to 12 months from the date of receipt.

Target

Background

The G-protein coupled receptor for 5-hydroxytryptamine (serotonin) is also involved in responding to various drugs and substances that affect the mind. When a molecule binds to this receptor, it changes its shape and activates signaling through G proteins, which then modulates the activity of downstream molecules like adenylate cyclase. The family of beta-arrestin proteins can halt signaling through G proteins and instead activate alternative signaling pathways. This signaling inhibits the activity of adenylate cyclase and triggers a system that regulates the release of calcium ions from within the cells. This receptor is important in controlling the release of 5-hydroxytryptamine and in regulating the metabolism of both dopamine and 5-hydroxytryptamine. Consequently, it influences neural activity, mood, and behavior. Moreover, it plays a role in the response to anxiety-inducing stimuli.

Tissue specificity

Detected in lymph nodes, thymus and spleen. Detected in activated T-cells, but not in resting T-cells.

Celluar localization

Plasma Membrane;

UniPort

P08908