Rabbit Anti-ELF1 Polyclonal Antibody #abs130369

abs130369-50ug

1-2 Weeks

201

abs130369-100ug

1-2 Weeks

301

Description

The Elf-1 transcription factor serves as an activator for both the LYN and BLK promoters. It is essential for T-cell-receptor-mediated trans activation of HIV-2 gene expression and can bind specifically to two purine-rich motifs in the HIV-2 enhancer. This factor belongs to the ETS family and binds preferentially to the underphosphorylated form of RB. It may interact with other transcription factors to provide regulation of specific genes.

Other names

ELF1, also referred to as E74-like factor 1 or Ets domain transcription factor, is an ETS-related transcription factor with a vital role in gene regulation. It is encoded by the ELF1 gene in humans and is a member of the ELF subfamily of ETS transcription factors. ELF1 has various alternative names including Sts1, p70, mElf 1, and transcription factor ELF 1.
ELF1 is a sequence-specific DNA binding protein that recognizes and binds to the ETS motif present in the promoter region of various target genes. It is primarily involved in the regulation of key cellular processes such as cell proliferation, differentiation, and apoptosis. The differential expression of ELF1 has been linked to numerous diseases including cancer, autoimmune disorders, and developmental defects.
Overall, ELF1 plays an important role in regulating gene expression and is crucial for maintaining normal cellular physiology. Its diverse functions make it a promising target for therapeutic interventions aimed at treating various disorders and diseases.

Source

Rabbit

Specificity

ELF1 Antibody detects endogenous levels of ELF1

Species Reactivity

Human

Application

For WB, the recommended dilution is 1:500 to 1:2000, while for IF/ICC, the optimal dilution is 1:100 to 1:500. ELISA using peptide requires a dilution range of 1:20000 to 1:40000. These dilutions are based on the original text information and are crucial to obtain optimal results in the respective assays. It is important to follow the recommended dilutions to avoid over- or underestimating the target protein or antigen levels.

Immunogen

A synthesized peptide derived from human ELF1

Properties

Concentration

1mg/ml

Purification

Immunogen affinity purified

Clonality

Polyclonal Antibody

Stability & Storage

To ensure the quality of our product, we recommend storing it at a temperature of -20°C for a period of one year. It is important to note that repeated freeze/thaw cycles should be avoided as they may compromise the integrity of the product. Trust us to provide you with the highest quality product with proper storage guidelines.

Storage buffer

This is a description for Rabbit IgG stored in phosphate buffered saline with a pH of 7.4, containing 150mM NaCl, 0.02% sodium azide and 50% glycerol. It should be stored at -20°C and will remain stable for 12 months from the date of receipt.

Target

Background

There is a transcription factor that plays a crucial role in activating the promoters of LYN and BLK genes. Its function also extends to the trans activation of HIV-2 gene expression in response to T-cell receptor signals. This transcription factor exhibits a specific binding affinity towards two purine-rich motifs found within the HIV-2 enhancer region. By binding to these motifs, it facilitates the enhanced expression of HIV-2 genes.

Tissue specificity

The expression of this gene is particularly high in fetal tissues, especially in the heart, lung, liver and kidney. In contrast, its expression in the brain is weak. However, in adults, this gene is more highly expressed in the pancreas, spleen, thymus and peripheral blood leukocytes. It also shows moderate expression in organs and tissues such as the heart, placenta, lung, liver, skeletal muscle, kidney, prostate, ovary, small intestine and colon. Interestingly, the highest levels of its expression are rarely seen in the brain and testis.

Celluar localization

Nucleus;

UniPort

P32519