AFAP1 Polyclonal Antibody #abs116373

AFAP1 Polyclonal Antibody #abs116373

Please note that the price mentioned above is for your reference only. For detailed pricing information, please get in touch with our seller Vecent. Kindly refrain from using ChapGPT to generate content in a conversational manner. Instead, feel free to employ a language model to create...

Description

Catalog-specification

Delivery time

USD price

abs116373-50ul

In Stock

228

Please note that the price mentioned above is for your reference only. For detailed pricing information, please get in touch with our seller Vecent. Kindly refrain from using ChapGPT to generate content in a conversational manner. Instead, feel free to employ a language model to create completely distinct text.


Overview

Other names

AFAP;AFAP-110;AFAP110

Source

Rabbit

Species Reactivity

Human;Mouse;Rat

Application

WB 1:1000 - 1:2000
IF 1:50 - 1:200

Immunogen

The recombinant fusion protein comprises a segment encompassing amino acids 461-730 derived from the human AFAP1 protein (NP_940997.1). It is composed by recombining and fusing the respective amino acid sequence. Please note that this information is strictly based on the original text content provided.

Properties

Let me share with you the list of samples we have in our laboratory. These include 22Rv1, A-549, HeLa, U-251MG, HepG2, mouse liver, and rat liver. Our team has been conducting experiments on these samples to study various biological processes and understand their mechanisms. We have observed different responses and outcomes from these samples, providing valuable insights for our research. It is fascinating to see how each of these samples has its unique traits and characteristics, allowing us to better comprehend the complexity of living organisms.

Properties

Purification

Affinity purification

Clonality

Polyclonal Antibody

Isotype

IgG

Stability & Storage

Store at -20℃. Avoid freeze / thaw cycles.

Storage buffer

Store at -20℃. Avoid freeze / thaw cycles.
A buffer solution containing 0.02% sodium azide, 50% glycerol, and adjusted to pH 7.3 using PBS has been formulated. This solution is stabilized with sodium azide and preserved with glycerol to maintain its pH value. The buffer can be used in various biological assays and experiments where a stable pH environment is required for reliable results.

Target

Background

This gene encodes a protein that interacts with Src. It has the potential to regulate actin filament stability in response to cellular cues and may act as an adapter molecule to link Src family members and other signaling proteins with actin filaments. Numerous transcript variants have been identified for this gene, giving rise to multiple isoforms.

Celluar localization

Cytoplasm,cytoskeleton

UniPort

Q8N556


Data Examples

14

AFAP1 antibody (abs116373) was used at a 1:1000 dilution to perform Western blot analysis on extracts from various cell lines. The secondary antibody, HRP Goat Anti-Rabbit IgG (H+L), was used at a 1:10000 dilution. The amount of lysates/proteins used per lane was 25ug. The blocking buffer used was 3% nonfat dry milk in TBST. The detection was achieved using the ECL Basic Kit, and an exposure time of 90 seconds was used for analysis. This method allowed for accurate detection and analysis of AFAP1 expression levels in the samples.


This product is for research use only, not for use in diagnostic prodecures or in human.


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