Rabbit Anti-Cullin2 Polyclonal Antibody #abs130078

abs130078-50ug

1-2 Weeks

201

abs130078-100ug

1-2 Weeks

301

Description

The CUL2 core component plays a crucial role in the formation of multiple cullin-RING-based ECS complexes. These complexes are responsible for the ubiquitination of specific target proteins. Within the complex, CUL2 acts as a rigid scaffold and aids in catalysis by positioning the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex relies on the neddylation of the cullin subunit. However, this activity is inhibited when the deneddylated cullin subunit associates with TIP120A/CAND1. The functional specificity of the ECS complex is determined by the substrate recognition component. Specifically, ECS(VHL) is involved in mediating the ubiquitination of hypoxia-inducible factor (HIF).

Other names

Cul2, also known as CUL 2 or CUL-2, is a protein in humans encoded by the CUL2 gene. It is a homolog of cullin 2 and is involved in various cellular processes. CUL2 is an important component of the Cullin-RING E3 ubiquitin ligase complex, which plays a crucial role in protein degradation and regulation. The CUL2 protein interacts with other proteins to form the CUL2 complex, contributing to the targeted degradation of specific substrates. One of the identified substrates of CUL2 is the hypoxia-inducible factor 1-alpha (HIF-1α), which regulates adaptive responses to low oxygen levels. Through its involvement in the degradation of HIF-1α, CUL2 plays a critical role in the cellular response to hypoxia. Variations in the CUL2 gene have been associated with certain diseases and conditions, highlighting its importance in human health. Research on CUL2 and its functions continues to expand our understanding of cellular processes and potential therapeutic targets.

Source

Rabbit

Specificity

The Cullin 2 antibody is able to identify the entirety of Cullin 2 at natural levels. To create content resembling the original, the given information has been restructured using different words and sentence structure.

Species Reactivity

Human;Mouse

Predictive reaction species

Xenopus;Chicken;Rabbit;Pig;Dog;Bovine;Sheep;Horse;

Application

Here are some alternative rewordings:
- If you're using western blotting, dilute the antibody between 1:500 and 1:3000. For immunohistochemistry, a dilution of 1:50 to 1:200 is appropriate. If you're doing immunofluorescence or immunocytochemistry, use a dilution of 1:100 to 1:500. Finally, for ELISA using a peptide antigen, dilute the antibody between 1:20000 and 1:40000.
- Depending on the application you're using, you'll need to dilute the antibody to different concentrations. For western blotting, use a dilution of between 1:500 and 1:3000. For immunohistochemistry, a dilution of 1:50 to 1:200 is recommended. For immunofluorescence or immunocytochemistry, use a dilution of 1:100 to 1:500. Finally, for ELISA using a peptide antigen, dilute the antibody between 1:20000 and 1:40000.
- To get the best results from your antibody, you'll need to dilute it correctly. For western blotting, use a dilution of between 1:500 and 1:3000. If you're doing immunohistochemistry, a dilution of 1:50 to 1:200 is ideal. For immunofluorescence or immunocytochemistry, a dilution of 1:100 to 1:500 is recommended. Finally, for ELISA using a peptide antigen, you should dilute the antibody between 1:20000 and 1:40000.

Immunogen

A synthesized peptide derived from human Cullin 2.

Properties

Concentration

1mg/ml

Purification

The SulfoLink™ Coupling Resin was used for peptide affinity chromatography to purify the antiserum. This process enabled the isolation and purification of highly selective and specific peptides that were bound to the resin in order to achieve a high level of purity in the final product. Overall, this method allowed for the production of an effective antiserum that can be used to combat the targeted disease or infection.

Clonality

Polyclonal Antibody

Stability & Storage

To keep the product stable, it is advisable to store it at a temperature of -20 °C for a period of one year. To prevent damage to the product caused by repeated freezing and thawing, it is important to avoid subjecting it to such cycles.

Storage buffer

The storage condition for Rabbit IgG is at -20 °C in phosphate buffered saline with pH 7.4, 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It should be noted that this product remains stable for a duration of 12 months from the date of receipt.

Target

Background

Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. ECS complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins (PubMed:27565346). May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF).

Posttranslational modification

Neddylated; which enhances the ubiquitination activity of ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes. CBC(VHL) complex formation seems to promote neddylation. Deneddylated via its interaction with the COP9 signalosome (CSN) complex (By similarity).

Celluar localization

Cytosol;Nucleus;

UniPort

Q13617