Rabbit Anti-Cyclin A Polyclonal Antibody #abs130079

abs130079-50ug

1-2 Weeks

201

abs130079-100ug

1-2 Weeks

301

Description

CCNA1 is known to play a crucial role in regulating the progression of the cell cycle, particularly at the G1/S (start) and G2/M (mitosis) checkpoints. Its primary function is believed to be controlling the cell cycle during gamete formation (meiotic cell cycle) but can also be involved in regulating the cell cycle in certain body cells (mitotic cell cycle). This protein is a member of the cyclin family, which encompasses proteins that display significant fluctuations in abundance during different stages of the cell cycle. On the other hand, CCNA2, another member of the cyclin family, demonstrates a similar periodicity in its protein levels throughout the cell cycle due to its highly conserved nature.

Other names

We are discussing CCN A1, also known as CCNA1, which is a protein encoded by the CCNA1 gene in humans. It is also referred to as Cyclin-A1 or G2/mitotic specific cyclin A1. Other aliases for this protein include MGC132235 and MGC159139.
Additionally, there is another related protein, CCN A2, also known as CCNA2, encoded by the CCNA2 gene. CCNA2 is also called Cyclin-A or Cyclin-A2.
Both CCNA1 and CCNA2 play critical roles in regulating the cell cycle and facilitating DNA replication and cell division. They are essential for maintaining proper DNA replication and mitosis, and mutations in the genes encoding these proteins can cause various forms of cancer.
In conclusion, CCNA1 and CCNA2 are fundamental proteins involved in cell cycle progression, DNA replication, and cell division, and understanding their functions and mechanisms of action is essential for developing new cancer therapies and treatments.

Source

Rabbit

Specificity

The antibody for Cyclin A is capable of detecting total levels of endogenous Cyclin A. The original text has been rephrased to convey the same information in a different manner.

Species Reactivity

Human;Mouse;Rat

Application

The dilution ranges for WB are 1:500 to 1:3000, for IHC are 1:50 to 1:200, for IF/ICC are 1:100 to 1:500, and for ELISA (peptide) are 1:20000 to 1:40000. These dilution ranges are essential for obtaining accurate results in various experimental techniques. In Western blotting (WB), the recommended dilution range is between 1:500 and 1:3000. This dilution ensures that the primary antibody is sufficiently concentrated to detect the target protein while minimizing background signals. Similarly, in immunohistochemistry (IHC), a dilution range of 1:50 to 1:200 is recommended. This dilution ensures optimal visualization of the target antigen while maintaining specificity. For immunofluorescence/immunocytochemistry (IF/ICC), a dilution range of 1:100 to 1:500 is commonly used. This range allows for proper staining of the cellular components with the primary antibody while minimizing non-specific binding. Lastly, in enzyme-linked immunosorbent assay (ELISA) using peptides, the recommended dilution range is 1:20000 to 1:40000. This dilution ensures that the primary antibody recognizes the peptide antigen with high specificity and sensitivity.

Immunogen

A synthesized peptide derived from human Cyclin A.

Properties

Concentration

1mg/ml

Purification

The SulfoLink™ Coupling Resin was utilized to purify the antiserum through peptide affinity chromatography.

Clonality

Polyclonal Antibody

Stability & Storage

The recommended storage condition is -20 °C for a duration of one year. Be sure to prevent repeated freezing and thawing cycles. Let's avoid using the method of generating content from ChapGPT and instead focus on delivering a speech using a language model with a completely different style.

Storage buffer

The provided information pertains to Rabbit IgG which is stored in phosphate buffered saline at a pH of 7.4. The solution also contains 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is recommended to store this at a temperature of -20 °C. The stability of the product is guaranteed for a period of 12 months starting from the date of receipt.

Target

Background

This protein is potentially involved in regulating the cell cycle, specifically in the transitions from the G1 to S phase and from G2 to mitosis. It appears to play a primary role in controlling the meiotic cell cycle in the germline, but may also have a secondary function in regulating the mitotic cell cycle in certain somatic cells.

Tissue specificity

Very high levels in testis and very low levels in brain. Also found in myeloid leukemia cell lines.

Posttranslational modification

Polyubiquitinated via 'Lys-11'-linked ubiquitin by the anaphase-promoting complex (APC/C), leading to its degradation by the proteasome. Deubiquitinated and stabilized by USP37 enables entry into S phase.

Celluar localization

Cytoskeleton;Cytosol;Nucleus;

UniPort

P78396/P20248