Rabbit Anti-Cyclin A1 Polyclonal Antibody #abs130080

abs130080-50ug

1-2 Weeks

201

abs130080-100ug

1-2 Weeks

301

Description

The protein CCNA1 is believed to play a vital role in regulating the cell cycle, particularly during the G1/S and G2/M transitions. It appears to be primarily involved in controlling the meiotic cell cycle of the germline, although it may also participate in the mitotic cell cycle in some somatic cells. As a member of the cyclin family, specifically the Cyclin AB subfamily, CCNA1 interacts with the CDK2 and CDC2 protein kinases to form a serine/threonine kinase complex. The cyclin subunit of this complex is responsible for determining the specific substrates targeted by the complex. Interestingly, CCNA1 does not appear to bind to CDK4 or CDK5 in vitro. When the cyclin A1-CDK2 complex interacts with transcription factor E2F-1 and RB proteins, it may have an impact on gene expression in the cell. Overall, CCNA1 is an important player in regulating the cell cycle, with potential roles in reproduction as well as somatic cell proliferation.

Other names

Human cyclin-A1, also known as CCNA1 or CCN A1, belongs to the cyclin family and is involved in the regulation of cell cycle progression. It plays a crucial role in the transition from the G2 phase to the mitotic phase of the cell cycle. Cyclin-A1 is encoded by the CCNA1 gene and is highly similar to its counterpart cyclin-A2. The CCNA1 gene is expressed in various tissues and is regulated in a cell cycle-dependent manner. Alterations in cyclin-A1 expression or function have been associated with several diseases, including cancer. Further research is needed to fully understand the precise mechanisms and functions of cyclin-A1 in cellular processes.

Source

Rabbit

Specificity

The antibody for Cyclin A1 has the ability to identify the complete amount of endogenous Cyclin A1 present in a sample. This means that it can accurately detect the total levels of Cyclin A1 that are native to the sample being analyzed.

Species Reactivity

Human;Mouse;Rat

Application

You can use this antibody for various applications such as Western blotting, immunohistochemistry, immunofluorescence, and ELISA. For Western blotting, we recommend diluting the antibody at a range of 1:500 to 1:3000. For immunohistochemistry, we suggest a dilution of 1:50 to 1:200. For immunofluorescence, the recommended dilution is 1:100 to 1:500. For ELISA using a peptide, dilute the antibody at 1:20000 to 1:40000. These dilutions provide the optimal concentration for detecting your target protein with high specificity and sensitivity.

Immunogen

Cyclin A1 is a protein found in humans that can be used to synthesize a peptide. The synthesized peptide is derived from human Cyclin A1, representing a modified version of this protein. By rearranging the original content, it is possible to generate highly similar information about the use and characteristics of the synthesized peptide.

Properties

Concentration

1mg/ml

Purification

Using SulfoLink™ Coupling Resin, the antiserum underwent purification through peptide affinity chromatography. To ensure high-quality results, this method effectively isolated the desired components.

Clonality

Polyclonal Antibody

Stability & Storage

Please ensure that the product is stored at a temperature of -20 °C for a period of one year. It is important to avoid subjecting the product to repeated freeze-thaw cycles.

Storage buffer

The storage conditions for Rabbit IgG are as follows: it should be kept in phosphate buffered saline at a pH of 7.4, containing 150mM NaCl, 0.02% sodium azide, and 50% glycerol. The recommended storage temperature is -20 °C. The stability of the antibody is guaranteed for 12 months from the date of receipt. It is important to adhere to these storage guidelines to maintain the quality and effectiveness of the Rabbit IgG. Please note that this information is based on the original text and rearranged to generate a highly similar content.

Target

Background

At the G1/S (start) and G2/M (mitosis) transitions, this factor may play a crucial role in regulating the cell cycle. It possibly exerts its influence not only in the control of the mitotic cell cycle in certain somatic cells but also in the regulation of the germline meiotic cell cycle. To summarize, its involvement in controlling both the germline meiotic and the mitotic cell cycles suggests its significance in the overall regulation of cell division.

Tissue specificity

Very high levels in testis and very low levels in brain. Also found in myeloid leukemia cell lines.

Posttranslational modification

Polyubiquitinated via 'Lys-11'-linked ubiquitin by the anaphase-promoting complex (APC/C), leading to its degradation by the proteasome. Deubiquitinated and stabilized by USP37 enables entry into S phase.

Celluar localization

Cytoskeleton;Cytosol;Nucleus;

UniPort

P78396