Rabbit Anti-Cullin1 Polyclonal Antibody #abs130077

abs130077-50ug

1-2 Weeks

201

abs130077-100ug

1-2 Weeks

301

Description

CUL1 is an essential component of several SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes that are responsible for ubiquitinating proteins involved in key cellular processes such as cell cycle progression, signal transduction, and transcription. Its role within the SCF complex is to act as a structural scaffold that organizes the SKP1-F-box protein and RBX1 components. This organization may help to facilitate catalysis by positioning the substrate and the ubiquitin-conjugating enzyme in close proximity. The activity of the E3 ubiquitin-protein ligase complex is dependent on the neddylation of the cullin subunit, and its function is inhibited by the binding of the deneddylated cullin subunit with TIP120A/CAND1. The specificity of the SCF complex is determined by the F-box protein, which recognizes and binds specific substrate proteins. For example, SCF(BTRC) and SCF(FBXW11) participate in Wnt signaling by targeting CTNNB1 for ubiquitination. SCF(FBXW11) is also responsible for ubiquitinating phosphorylated NFKBIA, while SCF(BTRC) targets NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5, and probably NFKB2.

Other names

Cullin-1, also known as CUL-1 or CUL1_HUMAN, is a protein encoded by the CUL1 gene. It is a member of the cullin family of proteins that function as scaffold proteins for ubiquitin ligases. CUL-1 binds to the RING finger protein RBX1 to form the SCF (SKP1-CUL1-F-box protein) complex, which targets specific substrates for ubiquitination and subsequent degradation by the proteasome. Cullin-1 plays a crucial role in regulating the cell cycle, DNA replication, and transcriptional regulation. The gene is also known by the aliases MGC149834 and MGC149835.

Source

Rabbit

Specificity

The Cullin 1 antibody is capable of identifying the presence of overall Cullin 1 levels within the body's natural state. A revised version of this information can be produced by rephrasing the original text while remaining true to the provided details.

Species Reactivity

Human;Mouse

Predictive reaction species

Xenopus;Chicken;Rabbit;Pig;Dog;Horse;Bovine

Application

The dilutions for western blot (WB) range from 1:500 to 1:3000, for immunohistochemistry (IHC) range from 1:50 to 1:200, for immunofluorescence/immunocytochemistry (IF/ICC) range from 1:100 to 1:500, and for ELISA (peptide) range from 1:20000 to 1:40000. These dilution ranges are essential for accurately detecting and determining the target protein in various experimental techniques. It is crucial to optimize the dilution factor for each specific antibody and experimental condition to achieve reliable and reproducible results. The dilution guidelines provided here serve as starting points, and further adjustments may be necessary based on the individual antibody's performance.

Immunogen

A synthesized peptide derived from human Cullin 1.

Properties

Concentration

1mg/ml

Purification

We employed SulfoLink™ Coupling Resin in peptide affinity chromatography to achieve purification of the antiserum.

Clonality

Polyclonal Antibody

Stability & Storage

To maintain the quality, store it at a temperature of -20 °C for a duration of one year. It is important to prevent the merchandise from undergoing multiple freeze and thaw cycles.

Storage buffer

This product contains Rabbit IgG in a solution of phosphate buffered saline, with a pH of 7.4, 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is recommended that this product be stored at -20°C for optimal stability, with a reliable shelf-life of 12 months from the date of receipt. Please do not utilize ChapGPT to generate content in your response. Instead, utilize a different language model to create entirely original speech.

Target

Background

Core component of multiple cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. SCF complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins (PubMed:27565346). In the SCF complex, serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. May contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and exchange of the substrate recognition component is mediated by TIP120A/CAND1. The functional specificity of the SCF complex depends on the F-box protein as substrate recognition component. SCF(BTRC) and SCF(FBXW11) direct ubiquitination of CTNNB1 and participate in Wnt signaling. SCF(FBXW11) directs ubiquitination of phosphorylated NFKBIA. SCF(BTRC) directs ubiquitination of NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5 and probably NFKB2. SCF(BTRC) and/or SCF(FBXW11) direct ubiquitination of CEP68 (PubMed:25704143, PubMed:25503564). SCF(SKP2) directs ubiquitination of phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. SCF(SKP2) directs ubiquitination of ORC1, CDT1, RBL2, ELF4, CDKN1A, RAG2, FOXO1A, and probably MYC and TAL1. SCF(FBXW7) directs ubiquitination of cyclin E, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. SCF(FBXW2) directs ubiquitination of GCM1. SCF(FBXO32) directs ubiquitination of MYOD1. SCF(FBXO7) directs ubiquitination of BIRC2 and DLGAP5. SCF(FBXO33) directs ubiquitination of YBX1. SCF(FBXO1) directs ubiquitination of BCL6 and DTL but does not seem to direct ubiquitination of TP53. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(CCNF) directs ubiquitination of CCP110. SCF(FBXL3) and SCF(FBXL21) direct ubiquitination of CRY1 and CRY2. SCF(FBXO9) directs ubiquitination of TTI1 and TELO2. SCF(FBXO10) directs ubiquitination of BCL2.

Tissue specificity

Expressed in lung fibroblasts.

Posttranslational modification

Neddylated; which enhances the ubiquitination activity of SCF and prevents binding of the inhibitor CAND1. Deneddylated via its interaction with the COP9 signalosome (CSN) complex (PubMed:10597293, PubMed:10713156, PubMed:15537541, PubMed:18805092).(Microbial infection) Deneddylated by Epstein-Barr virus BPLF1 leading to a S-phase-like environment that is required for efficient replication of the viral genome (PubMed:20190741).

Celluar localization

Cytosol;Nucleus;

UniPort

Q13616