Rabbit Anti-PGP9.5 Polyclonal Antibody #abs130179

abs130179-50ug

1-2 Weeks

201

abs130179-100ug

1-2 Weeks

301

Description

The UCHL1 protein is an important player in the regulation of ubiquitin levels within cells. It acts as both a processor of ubiquitin precursors and a hydrolase for ubiquitinated proteins. Through its thiol protease activity, UCHL1 can specifically recognize and cleave the peptide bond at the C-terminal glycine of ubiquitin. This process is critical for the maintenance of proper ubiquitin homeostasis and the regulation of various cellular processes.

Other names

Protein gene product 9.5, also known as PGP 9.5 or PGP9.5, is a luminal protein found in the epididymis. It is encoded by the UCHL1 gene and belongs to the ubiquitin C-terminal hydrolase family. UCH-L1 plays a role in the regulation of protein turnover and the recycling of ubiquitin. It is also involved in neuronal function, as it is found in the cytoplasm of neurons. Additionally, UCH-L1 is associated with certain diseases, including Parkinson's disease (Park 5) and neurodegenerative disorders. Overall, UCH-L1 is an important protein with various functions in the human body.

Source

Rabbit

Specificity

The presence of total PGP9.5 can be identified using the PGP9.5 antibody, which is capable of detecting its endogenous levels. To maintain the original information, the rearranged content is as follows: "Detection of endogenous total PGP9.5 can be achieved using the PGP9.5 antibody."

Species Reactivity

Human;Mouse;Rat

Application

For Western Blot, a dilution of 1:500~1:3000 is recommended, while for Immunohistochemistry, a dilution range of 1:50~1:200 is suitable. For Immunofluorescence/Immunocytochemistry, a dilution range of 1:100-1:500 is recommended. When performing ELISA with peptide, it is suggested to use a dilution range of 1:20000-1:40000. It is important to note that these dilution ranges are based on the original text information and should be followed accordingly for optimal results.

Immunogen

A synthesized peptide derived from human PGP9.5.

Properties

Concentration

1mg/ml

Purification

Using SulfoLink™ Coupling Resin, the antiserum underwent peptide affinity chromatography to achieve purification. The resulting product was highly refined and of exceptional quality.

Clonality

Polyclonal Antibody

Stability & Storage

To maintain optimal conditions, the storage temperature should be kept at -20 °C for a duration of one year. It is important to prevent the occurrence of repeated freeze/thaw cycles. Please note that this arrangement is crucial for preserving the quality of the stored items.

Storage buffer

This rabbit IgG solution is formulated in phosphate buffered saline with a pH of 7.4. It contains 150mM of NaCl and 0.02% of sodium azide, along with 50% glycerol for stabilization. It is recommended to store this product at -20°C, and it is stable for 12 months from the date of receipt.

Target

Background

This thiol protease enzyme, known as ubiquitin-protein hydrolase, plays a crucial role in both the processing of ubiquitin precursors and the breakdown of ubiquitinated proteins. It specifically targets and cleaves the peptide bond located at the C-terminal glycine of ubiquitin. Additionally, it has the ability to bind to free monoubiquitin, potentially safeguarding it from degradation within lysosomes. Interestingly, the enzyme forms a homodimer and may also exhibit ATP-independent ubiquitin ligase activity. This enzyme's multifaceted functions make it an essential player in the regulation of protein degradation and recycling processes.

Tissue specificity

Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients.

Posttranslational modification

O-glycosylated.

Celluar localization

Cytosol;Endoplasmic reticulum;Extracellular region or secreted;Nucleus;

UniPort

P09936