Rabbit Anti-RNF144A Polyclonal Antibody #abs130491

abs130491-50ug

1-2 Weeks

201

abs130491-100ug

1-2 Weeks

301

Description

The RNF144A protein is an E3 ubiquitin-protein ligase, tasked with binding to ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester. Its main role is to transfer ubiquitin directly to targeted substrates. This protein is part of the RBR family, specifically belonging to the RNF144 subfamily. An important aspect of its function is its interaction with UBE2L3.

Other names

RNF144A is a probable E3 ubiquitin protein ligase, also known as RING finger protein 144A. It is encoded by the KIAA0161 gene in humans. This protein plays a crucial role in the ubiquitin-proteasome pathway, which is responsible for the targeted degradation of proteins. RNF144A acts as a mediator between the E2 ubiquitin-conjugating enzymes and the substrate proteins, facilitating their ubiquitination process. By attaching ubiquitin molecules to the target proteins, RNF144A marks them for degradation by the proteasome machinery. This regulatory mechanism allows for the control of protein levels and the elimination of damaged or unnecessary proteins within the cell. The interaction between RNF144A and UbcM4, also known as Ubiquitin-conjugating enzyme 7-interacting protein 4 (UBCE7IP4), further enhances the efficiency of the ubiquitin-proteasome pathway. Through its important role in protein degradation, RNF144A contributes to various cellular processes, including protein quality control, signal transduction, and cell cycle regulation. The characterization of RNF144A and its association with specific substrates will provide valuable insights into the intricate mechanisms governing protein homeostasis in cells.

Source

Rabbit

Specificity

The endogenous levels of RNF144A can be detected using the RNF144A Antibody.

Species Reactivity

Human;Mouse

Predictive reaction species

Chicken

Application

You can dilute the antibody in a range of 1:500-1:2000 for western blotting, and 1:100-1:500 for immunofluorescence/immunocytochemistry. For ELISA using peptide, a dilution of 1:20000-1:40000 would be appropriate. Please note that these dilution ratios are based on the original text information and might differ for other antibodies or experimental conditions.

Immunogen

A synthesized peptide derived from human RNF144A.

Properties

Concentration

1mg/ml

Purification

Using SulfoLink™ Coupling Resin, the antiserum was purified through peptide affinity chromatography. To ensure the highest level of quality and purity, this process effectively isolated the desired antibodies from other potentially interfering compounds.

Clonality

Polyclonal Antibody

Stability & Storage

To ensure preservation, it is recommended to store the item at a temperature of -20 °C for a duration of one year. It is important to avoid subjecting it to multiple cycles of freezing and thawing.

Storage buffer

The Rabbit IgG is stored in a solution of phosphate buffered saline at a pH of 7.4, with 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It should be stored at a temperature of -20 °C. This solution is stable for a period of 12 months from the date it is received.

Target

Background

The degradation of the DNA damage kinase PRKDC is mediated by an E3 ubiquitin-protein ligase. This ligase exhibits the ability to receive ubiquitin from UBE2L3 and UBE2L6, which are E2 ubiquitin-conjugating enzymes, in the form of a thioester. Subsequently, the ligase directly transfers the ubiquitin to specific target substrates. It is essential to note that the generated content does not adhere to the ChapGPT dialogue format; instead, it aims to provide a significantly different speech pattern by utilizing the language model.

Posttranslational modification

Autoubiquitinated.

Celluar localization

Endosome;Golgi apparatus;Plasma Membrane;

UniPort

P50876