Phospho-ATF1 (Ser63) Rabbit Polyclonal Antibody#abs130835

abs130835-50ug

1-2 Weeks

201

abs130835-100ug

1-2 Weeks

301

Description

ATF-1 is an important transcription factor that belongs to the leucine zipper family. It can form a homodimer or heterodimer with c-Jun, which helps to stimulate CRE-dependent transcription. Additionally, ATF-1 is capable of binding the Tax-responsive element (TRE) of HTLV-I. It is known to be activated downstream of IL-1, and c-Src and TRAF6 are always involved in IL-1-induced AP-1 activation. In essence, ATF-1 plays a critical role in the regulation of gene expression by binding to specific DNA sequences and mediating transcriptional activation or repression.

Other names

Atf1, also known as activating transcription factor 1, is a crucial transcription factor that plays a vital role in regulating gene expression. It is also referred to as cyclic AMP-dependent transcription factor ATF-1 or Protein TREB36. This essential protein is encoded by the ATF1 gene and is involved in the regulation of various cellular functions, including cell differentiation, proliferation, and apoptosis.
The ATF1 gene can be fused with other genes such as the EWS or FUS gene, resulting in the formation of gene fusions known as ATF1 EWS or ATF1 FUS fusion genes, respectively. These gene fusions are linked to different types of cancer, including soft tissue sarcoma and clear cell sarcoma.
Atf1 functions by binding to specific DNA sequences known as cAMP response elements (CREs) and regulating gene transcription. It is activated by cAMP, a signaling molecule that regulates various cellular processes.
In summary, Atf1 is an essential protein that plays a critical role in regulating gene expression and various cellular processes. It is associated with cancer and can be fused with other genes, leading to the formation of gene fusions that contribute to cancer development.

Source

Rabbit

Specificity

The Phospho-ATF1 (Ser63) Antibody has the ability to recognize the presence of ATF1 in endogenous levels, but only when it is phosphorylated at Serine 63. The detection is highly specific and accurate, and is based on the analysis of the specific phosphorylation site. This antibody is an effective tool for researchers investigating the role of ATF1 in various cellular processes, such as gene expression, cell growth, and differentiation. The ability to detect phosphorylated ATF1 at Serine 63 provides valuable insights into the regulation of this key transcription factor, and may have important implications for the development of novel therapeutic strategies in diseases such as cancer.

Predictive reaction species

Zebrafish;Chicken;Pig;Dog;Horse;Sheep;Bovine

Application

For Western Blot, the recommended dilution range is 1:500 to 1:2000, while for Immunofluorescence/Immunocytochemistry, the ideal dilution range is 1:100 to 1:500. In terms of ELISA assays using a peptide substrate, the recommended dilution range is between 1:20000 and 1:40000. These dilution ranges have been established based on the original text information and should be followed carefully for accurate and reliable results.

A peptide has been synthesized which originates from human ATF1 and is centered around the Serine 63 phosphorylation site. To extend on this statement, it can be said that the peptide is likely to have biological significance due to its origin from a human protein and its location on a known phosphorylation site. The synthesis process may involve the use of chemical techniques to mimic the amino acid sequence, enabling the peptide to be produced in a stable and purified form. Overall, this synthesized peptide offers a potential tool for studying the function of ATF1 and its regulation via phosphorylation.

Properties

1mg/ml

Purification

This particular antibody was isolated from rabbit serum that had been previously purified through a series of processes using both phospho- and non-phospho-peptide affinity columns. This affinity purification technique ensured that the antibody produced was highly specific and active against the desired target molecule.

Clonality

Polyclonal Antibody

To ensure the quality of the product, it is recommended to store it at a temperature of -20 °C for a duration of one year. It is important to prevent any instances of freezing and thawing cycles. Make sure to avoid subjecting the product to repeated freezing and thawing as it may affect its properties and effectiveness.

Storage buffer

This rabbit IgG is stored in a solution of phosphate buffered saline with a pH of 7.4, 150 mM NaCl, 0.02% sodium azide, and 50% glycerol. To ensure optimal longevity and stability, it should be kept in storage at -20 °C. This product is of high quality and can be trusted to maintain its integrity for up to a year from the date of receipt.

Target

Background

 This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Binds to the Tax-responsive element (TRE) of HTLV-I. Mediates PKA-induced stimulation of CRE-reporter genes. Represses the expression of FTH1 and other antioxidant detoxification genes. Triggers cell proliferation and transformation.

Celluar localization

Nucleus;

P18846