Phospho-C/EBP- Beta (Thr235/188) Rabbit Polyclonal Antibody#abs130829

abs130829-50ug

1-2 Weeks

201

abs130829-100ug

1-2 Weeks

301

Description

This gene lacks introns and encodes a protein that functions as a bZIP transcription factor. It has the ability to bind to specific DNA regulatory regions, both as a homodimer and in partnership with other related proteins like CEBP-alpha, CEBP-delta, and CEBP-gamma.

Other names

There are many different names for CCAAT/enhancer-binding protein beta or C/EBP beta, an important transcription factor involved in the regulation of various cell processes. It is also sometimes referred to as AGP/EBP, C EBP beta, C/EBP related protein 2 (CRP2), IL 6DBP, Interleukin 6 dependent binding protein, LAP, Liver activator protein, Liver enriched transcriptional activator, NF IL6, Nuclear factor NF IL6, Nuclear factor NF-IL6, SF B, SFB, Silencer factor B, TCF-5, or Transcription factor 5. All of these terms refer to the same protein, and the various names reflect different aspects of its function and importance in the body. C/EBP beta plays a key role in the regulation of immune responses and inflammation, as well as in the development of various tissues and organs. Understanding its function and regulation is essential for developing new therapies for many different diseases and disorders.

Source

Rabbit

Specificity

The Antibody for Phospho-C/EBP- beta (Thr235/188) can specifically identify the level of endogenous C/EBP- beta when it is phosphorylated at Threonine 235/188. The phosphorylation status is essential for detecting the protein, and the antibody has been designed to provide high specificity and sensitivity. The test can generate accurate results for quantitative analysis and research applications. With its effectiveness in detecting the phosphorylated C/EBP- beta, the antibody is an essential tool for scientists studying the protein's role in different cellular processes.

Predictive reaction species

Rabbit;Pig;Dog;Bovine;

Application

The suggested dilutions for using this antibody are WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, and ELISA (peptide) 1:20000-1:40000. To obtain optimal results, it is recommended to determine the dilution factor for each specific application. Dilution factors that are too low or too high can result in poor signal-to-noise ratios and affect the sensitivity and specificity of the assay. Therefore, it is essential to optimize the dilution based on the sample type, assay conditions, and the antibody's affinity and specificity for the target protein. In summary, this antibody can be a valuable tool for various applications, and the suggested dilutions can be used as a starting point for developing an optimal protocol for a particular assay.

C/EBP-β235/188，，。

Properties

1mg/ml

Purification

By employing a sequential chromatography technique on phospho- and non-phospho-peptide affinity columns, we successfully purified the antibody from rabbit serum. This process involved using purified rabbit serum and subjecting it to affinity purification. The affinity purification allowed us to isolate the antibody by specifically binding it to the phospho- and non-phospho-peptide columns. Through this method, we obtained a highly pure antibody sample for further analysis and experimentation.

Clonality

Polyclonal Antibody

To maintain its quality, keep the store temperature at -20 °C for a duration of one year. It is crucial to prevent subjecting the product to multiple freeze/thaw cycles.

Storage buffer

The Rabbit IgG is stored in a solution of phosphate buffered saline with a pH of 7.4. The solution also contains 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is recommended to store the solution at -20 °C for optimum stability. The Rabbit IgG remains stable for a period of 12 months starting from the date of receipt.

Target

Background

 Important transcription factor regulating the expression of genes involved in immune and inflammatory responses (PubMed:1741402, PubMed:9374525, PubMed:12048245, PubMed:18647749). Plays also a significant role in adipogenesis, as well as in the gluconeogenic pathway, liver regeneration, and hematopoiesis. The consensus recognition site is 5'-T[TG]NNGNAA[TG]-3'. Its functional capacity is governed by protein interactions and post-translational protein modifications. During early embryogenesis, plays essential and redundant functions with CEBPA. Has a promitotic effect on many cell types such as hepatocytes and adipocytes but has an antiproliferative effect on T-cells by repressing MYC expression, facilitating differentiation along the T-helper 2 lineage. Binds to regulatory regions of several acute-phase and cytokines genes and plays a role in the regulation of acute-phase reaction and inflammation. Plays also a role in intracellular bacteria killing (By similarity). During adipogenesis, is rapidly expressed and, after activation by phosphorylation, induces CEBPA and PPARG, which turn on the series of adipocyte genes that give rise to the adipocyte phenotype. The delayed transactivation of the CEBPA and PPARG genes by CEBPB appears necessary to allow mitotic clonal expansion and thereby progression of terminal differentiation (PubMed:20829347). Essential for female reproduction because of a critical role in ovarian follicle development (By similarity). Restricts osteoclastogenesis (By similarity).

Tissue specificity

Expressed at low levels in the lung, kidney and spleen.

Celluar localization

Nucleus;

P17676