Phospho-Cyclin B1 (Ser126) Rabbit Polyclonal Antibody#abs130795

abs130795-50ug

1-2 Weeks

201

abs130795-100ug

1-2 Weeks

301

Description

Cyclins are an essential family of proteins that play a crucial role in regulating cell cycle progression. These proteins display remarkable periodicity in their abundance throughout the cell cycle. One discerning feature of cyclins is their intimate association with CDK kinases, forming a class of regulatory molecules that help modulate the timing and progression of cell division.

Other names

The protein CCNB1, also known as Cyclin B1 or G2/mitotic-specific cyclin-B1, plays an important role in the cell cycle. It is specifically expressed in the G2 phase and is required for entry into mitosis. CCNB1 forms a complex with cyclin-dependent kinase 1 (CDK1), which is necessary for progression through the G2/M checkpoint. Mutations in CCNB1 have been associated with various disease conditions, including cancer. Therefore, understanding the function and regulation of CCNB1 is crucial for the development of effective therapeutic strategies.

Source

Rabbit

Specificity

The Phospho-Cyclin B1 (Ser126) Antibody is capable of detecting Cyclin B1 at its natural levels, but only if it is phosphorylated on Serine 126. To provide similar information, the content can be said in a rearranged form. Cyclin B1 levels can be detected reliably with the help of Phospho-Cyclin B1 (Ser126) Antibody, but it can only detect those that have undergone phosphorylation on Serine 126.

Application

For Western Blotting, a dilution range of 1:500-1:2000 is suggested. Immunohistochemistry can be performed using a dilution range of 1:50-1:200. For Immunofluorescence or Immunocytochemistry, a dilution range of 1:100-1:500 is recommended. For ELISA assays using a peptide, a dilution range of 1:20000-1:40000 is optimal. It is important to ensure that appropriate dilution is used for each application to obtain accurate results.

A peptide that has been synthesized from the human Cyclin B1 protein and specifically targets the phosphorylation site of Serine 126 is now available. This peptide is highly similar in composition to the original protein, allowing for precise and targeted interactions with intracellular pathways involved in cell division and cell cycle progression. Through the use of advanced techniques and careful molecular design, this peptide has been optimized for optimal bioavailability and efficacy, making it an indispensable tool for researchers studying cell biology and cancer. So if you're looking for a reliable way to study the intricate mechanisms of cell division and differentiation, look no further than this synthesized Cyclin B1 peptide.

Properties

1mg/ml

Purification

A highly specific antibody was obtained from rabbit serum by using a purification method that involved sequential chromatography on both phospho- and non-phospho-peptide affinity columns. The resulting product was highly purified and exhibited a high degree of similarity to the original antigen. This antibody is expected to be useful for a range of applications in both research and diagnostics. The affinity purification process ensured that only the desired antibody was obtained and that it was of high quality and purity. Overall, this antibody represents a valuable tool for the scientific community and will likely find widespread use in various areas of biomedical research.

Clonality

Polyclonal Antibody

To ensure the best storage conditions, it is recommended to keep the product at -20°C for a duration of one year. It is crucial to avoid subjecting it to repeated freeze/thaw cycles.

Storage buffer

This product contains Rabbit IgG in phosphate buffered saline at pH 7.4, with the addition of 150mM NaCl, 0.02% sodium azide, and 50% glycerol. For optimal storage, please keep at -20 °C. This product is stable for up to 12 months from the day of purchase.

Target

Background

At the G2/M transition, it is crucial to have proper control of the cell cycle. This control is essential to ensure that mitosis occurs in an organized and accurate manner. Without proper regulation, mistakes can occur, leading to genetic abnormalities and potentially harmful outcomes. Therefore, it is imperative to have a robust mechanism in place that governs the transition from G2 to mitosis, safeguarding the integrity of the genetic material and ensuring the proper development and function of the organism.

Tissue specificity

During G2, it gradually builds up and is suddenly eradicated during mitosis.

Celluar localization

Cytoskeleton;Cytosol;Mitochondrion;Nucleus;

P14635