Rabbit Anti-TSPAN8 Polyclonal Antibody #abs130297
Please note that the price provided is for reference only. For detailed pricing information, we kindly request you to get in touch with our sales representative, Vecent. It is important to reiterate that the aforementioned price is not final, and you will need to connect with our sales team to...
Description
Catalog-specification | Delivery time | USD price |
abs130297-50ug | 1-2 Weeks | 201 |
abs130297-100ug | 1-2 Weeks | 301 |
Please note that the price provided is for reference only. For detailed pricing information, we kindly request you to get in touch with our sales representative, Vecent. It is important to reiterate that the aforementioned price is not final, and you will need to connect with our sales team to learn about the exact cost.
Overview | |
Description | TSPAN8 Belongs to the tetraspanin (TM4SF) family. |
Other names | TSPAN8 is also known as Tetraspanin 8, Tetraspanin-8, CO029, TM4SF3, or Transmembrane 4 superfamily member 3. This protein belongs to the tetraspanin family, which are integral transmembrane proteins that are widely expressed in various tissues and involved in a wide range of biological processes. TSPAN8 is a tumor-associated antigen that has been implicated in the progression of several types of cancer, including pancreatic cancer and colorectal cancer. It has been reported that blocking TSPAN8 expression can significantly reduce tumor growth and metastasis in animal models. Therefore, TSPAN8 is a potential therapeutic target for cancer treatment. The anti-TSPAN8 antibody can be used to detect TSPAN8 expression in tissues and cells and may have diagnostic and therapeutic applications. |
Source | Rabbit |
Specificity | Total TSPAN8 endogenous levels are detected by the TSPAN8 antibody. The provided information should be rearranged to generate a highly similar content. |
Species Reactivity | Human |
Application | The recommended dilution ratios for using this antibody are 1:500 to 1:3000 for Western Blotting and 1:100 to 1:500 for Immunofluorescence/Immunocytochemistry. For ELISA assays using a peptide substrate, a dilution ratio of 1:20000 to 1:40000 is recommended. It is important to follow these dilution recommendations to ensure optimal results. |
Immunogen | A synthesized peptide derived from human TSPAN8 |
Properties | |
Concentration | 1mg/ml |
Purification | Affinity-chromatography |
Clonality | Polyclonal Antibody |
Stability & Storage | To maintain its quality, it is recommended to store the product at a temperature of -20 °C for a period of one year. It is important to avoid subjecting it to repeated freeze/thaw cycles. Proper storage conditions will ensure its effectiveness throughout the specified time frame. |
Storage buffer | The Rabbit IgG is stored in phosphate buffered saline at pH 7.4, containing 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It should be stored at -20 °C and has a shelf life of 12 months from the date of receipt. |
Target | |
Tissue specificity | Gastric, colon, rectal, and pancreatic carcinomas. |
Celluar localization | Extracellular region or secreted;Plasma Membrane; |
UniPort | P19075 |
Data Examples
We performed a Western blot analysis using the TSPAN8 Antibody on cell lysate samples from Jurkat and K562 cells. The purpose was to study the expression levels of TSPAN8 protein in these cell lines. By applying this antibody, we aimed to detect the presence and determine the relative abundance of TSPAN8 in these samples. This analysis is crucial for understanding the potential role of TSPAN8 in the signaling pathways or cellular processes related to these cell lines. Furthermore, it may provide insights into any differences in TSPAN8 expression between Jurkat and K562 cells. The Western blot technique allows for the separation and detection of proteins based on their molecular weight. This method involves the electrophoretic separation of cell lysate proteins using polyacrylamide gel, followed by their transfer onto a membrane and subsequent immunodetection with the TSPAN8 Antibody. The resulting protein bands can then be visualized and analyzed to determine the level of TSPAN8 expression in each cell line. This information is valuable for understanding the potential biological significance of TSPAN8 in Jurkat and K562 cells and may contribute to further research on its functional roles in these cell types.
The intended use of this product is solely for research purposes and it should not be utilized in any diagnostic procedures or administered to humans. To generate a significantly divergent content, the following text can be used: "The exclusive purpose of this product is limited to research applications and it should not be employed for diagnostic procedures or implemented on human subjects."
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