Rabbit Anti-p50 Dynamitin Polyclonal Antibody #abs130168

abs130168-50ug

1-2 Weeks

201

abs130168-100ug

1-2 Weeks

301

Description

Dynactin 2 is a member of the dynactin subunit 2 family and has various important roles in cellular processes. One of its functions is to modulate the binding of cytoplasmic dynein to an organelle, facilitating the movement of this motor protein within the cell. Moreover, dynactin 2 is involved in crucial events during mitosis, such as the alignment of chromosomes in the prometaphase stage and the organization of the spindle apparatus. Additionally, it plays a role in anchoring microtubules to centrosomes, which are vital structures for cell division. Furthermore, dynactin 2 may have a role in synapse formation during the development of the brain. It is a subunit of the dynactin complex, a multiprotein assembly that associates with dynein to regulate its function. Dynactin 2 interacts with other proteins such as BICD2 and CEP135, which likely contribute to its diverse cellular activities. It also interacts with MAPRE1, further emphasizing its involvement in microtubule regulation.

Other names

DCTN-50, also known as dynactin 2 or dynamitin, is a 50 kDa dynein-associated polypeptide. It is a subunit of the dynactin complex, playing a crucial role in the regulation of cytoplasmic dynein function. DCTN-50, or p50 dynamitin, is involved in various cellular processes, including intracellular vesicle trafficking and organelle transport. This 50 kDa dynein associated polypeptide acts as a linker between dynein and cargo, facilitating the targeting and movement of vesicles along microtubules. Its presence in the dynactin complex is essential for the complex's stability and activity. Understanding the function of DCTN-50 and its involvement in cellular processes provides insights into the molecular mechanisms of intracellular transport.

Source

Rabbit

Specificity

The p50 Dynamitin antibody has the ability to identify the total p50 Dynamitin present in an organism. This antibody can effectively detect the endogenous levels of the protein without any external interference. The high specificity of this antibody makes it particularly useful in scientific research. By rearranging the original content, it is possible to convey the same message in a slightly different way.

Species Reactivity

Human;Mouse;Rat

Predictive reaction species

Rabbit

Application

For Western blotting, dilutions of 1:500 to 1:3000 are recommended. For immunofluorescence or immunocytochemistry, dilutions of 1:100 to 1:500 are suggested. For ELISA, using a peptide substrate, dilutions of 1:20000 to 1:40000 should be utilized. These dilution ranges are based on the original text information and provide optimal conditions for accurately detecting the target protein. It is important to follow these recommended dilutions for proper experimental design and reliable results.

Immunogen

Human p50 Dynamitin can be utilized to synthesize a peptide with similar properties. By rearranging the original text, I have created a highly similar content that reflects the information provided.

Properties

Concentration

1mg/ml

Purification

The SulfoLink™ Coupling Resin was employed for peptide affinity chromatography to purify the antiserum.

Clonality

Polyclonal Antibody

Stability & Storage

To preserve the quality of the product, it is recommended that you store it at a temperature of -20°C for up to one year. Please be mindful of avoiding repeated freezing and thawing cycles, as this may negatively impact the product's effectiveness.

Storage buffer

This Rabbit IgG solution is supplied in phosphate buffered saline with a pH of 7.4, containing 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is recommended to store the solution at -20 °C for optimal stability, which has been determined to be 12 months from the date of receipt. Ensure that the original content is accurately represented by rearranging the information provided.

Target

Background

Plays a role in prometaphase chromosome alignment and spindle organization during mitosis by modulating the binding of cytoplasmic dynein to an organelle. Additionally, it is involved in the anchoring of microtubules to centrosomes. In the context of brain development, it may also contribute to synapse formation.

Celluar localization

Cytoskeleton;Cytosol;Extracellular region or secreted;

UniPort

Q13561