Phospho-Paxillin(Ser178) Rabbit Polyclonal Antibody #abs130021

abs130021-50ug

1-2 Weeks

201

abs130021-100ug

1-2 Weeks

301

Description

Paxillin (PXN) is a versatile protein found in various cellular domains that primarily localizes to the interfaces between cells and the extracellular matrix known as focal adhesion sites. It plays a crucial role in integrin signaling and is phosphorylated by focal adhesion kinase (FAK). This phosphorylation event facilitates the recruitment of signaling molecules to focal adhesions by creating docking sites. Specifically, the SH2 domain of Crk can bind to tyrosine phosphorylated paxillin, further enhancing its involvement in cellular signaling. Additionally, researchers have identified three different isoforms of paxillin that arise from alternative splicing.

Other names

The genes FLJ16691 and FLJ23042 both encode for proteins involved in cellular regulation and signaling. Specifically, FLJ16691 is responsible for producing the protein Paxillin alpha, which helps to control cellular adhesion and migration, while FLJ23042 codes for the Paired box protein Pax 1, also known as Paxillin. Both of these proteins play important roles in maintaining proper cellular function and are essential for various physiological processes.
Interestingly, the gene encoding for Paxillin alpha, PXN, is highly similar to the gene for Paxillin, PAX1, indicating that they may have developed from a common ancestor. This suggests that the two proteins may have overlapping functions and may work together to help regulate cellular behavior.
Overall, the identification and study of genes such as PXN and PAX1 is crucial for understanding the complex mechanisms that underlie cellular function and disease. By investigating these genes and their associated proteins, researchers can gain critical insights into the ways in which cells communicate and respond to their environment, ultimately leading to the development of new treatments and therapies for a wide range of disorders.

Source

Rabbit

Specificity

The Phospho-Paxillin(Ser178) Antibody specifically recognizes Paxillin protein in its phosphorylated state at Serine 178. It is capable of detecting the endogenous levels of phosphorylated Paxillin, allowing for the study of its role in various cellular processes.

Species Reactivity

Human;Mouse;Rat

Application

WB 1:500-1:2000, ELISA(peptide) 1:20000-1:40000

Immunogen

A novel peptide has been created using human Paxillin as a template, focusing on the Serine 178 phosphorylation site. The synthesized peptide is highly similar to the original structure and promises to have significant biological activity. This approach allows for the creation of new and potent peptides that can have a wide range of applications in various areas of research. With the development of advanced technologies, such as artificial intelligence and machine learning, we can expect to see more innovative and effective peptide designs in the coming years.

Properties

Concentration

1mg/ml

Purification

The purified rabbit serum was used to derive the antibody through a process of sequential chromatography on phospho- and non-phospho-peptide affinity columns. The affinity purification process ensured that the resulting antibody had a high degree of similarity to the original serum. The antibody is thus a highly specific and effective tool for detecting and identifying target peptides in a range of applications. This process represents a powerful approach for generating high-quality antibodies with exceptional specificity and sensitivity.

Clonality

Polyclonal Antibody

Stability & Storage

The recommended storage condition for this product is at a temperature of -20 °C for a duration of one year. It is important to avoid subjecting the product to repeated freeze/thaw cycles during this storage period.

Storage buffer

The Rabbit IgG is preserved in a solution of phosphate buffered saline, pH 7.4, containing 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is recommended to store this solution at a temperature of -20 °C. The product is stable for a duration of 12 months starting from the date of receipt. Please note that the requested text should be generated in a completely distinct manner from how ChapGPT would generate content.

Target

Background

Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).

Posttranslational modification

Phosphorylated by MAPK1/ERK2 (By similarity). Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens. Phosphorylation at Ser-244 by CDK5 reduces its interaction with PTK2/FAK1 in matrix-cell focal adhesions (MCFA) during oligodendrocytes (OLs) differentiation. Phosphorylation at Tyr-31 and Tyr-118 by PTK6 promote the activation of RAC1 via CRK/CrKII, thereby promoting migration and invasion. Phosphorylation at Ser-250 by SLK is required for PXN redistribution and cell motility (PubMed:23128389).

Celluar localization

Cytoskeleton;Cytosol;Plasma Membrane;

UniPort

P49023