Phospho-Retinoic Acid Receptor Alpha (Ser77) Rabbit Polyclonal Antibody #abs130019

abs130019-50ug

1-2 Weeks

201

abs130019-100ug

1-2 Weeks

301

Description

RARA, a member of the nuclear receptor superfamily, acts as a receptor for retinoic acid, a potent mammalian morphogen and teratogen with significant implications for vertebrate development. It exerts its control over cellular function by directly regulating the expression of genes. The phosphorylation of RARA is critically important for its transcriptional activity. Deviations involving this receptor may contribute to the onset of acute promyelocytic leukemia. The scientific community has identified two splice-variant isoforms of RARA, further expanding our understanding of its complexity and versatility.

Other names

Nuclear mitotic apparatus protein retinoic acid receptor alpha fusion protein, also known as NPM RAR long form or RAR alpha, is a member of the nuclear receptor subfamily 1 group B. It plays a crucial role in regulating gene expression and cellular processes. This fusion protein consists of two distinct domains, the nuclear mitotic apparatus protein and retinoic acid receptor alpha. It is involved in the binding of retinoic acid, a derivative of vitamin A, which activates the transcriptional activity of the protein. Through its interactions with various signaling pathways, NR1B1 influences cell growth, differentiation, and apoptosis. Moreover, it has been implicated in the development and progression of certain cancers, including acute promyelocytic leukemia. The multiple variants of retinoic acid receptor alpha, such as RARalpha1 and the two retinoic acid nuclear receptor alpha variants, exhibit slightly different structures and functions. Overall, the retinoic acid receptor alpha polypeptide is an essential factor in orchestrating cellular processes and signifies its significance in both normal and pathological conditions.

Source

Rabbit

Specificity

The Phospho-Retinoic Acid Receptor alpha (Ser77) Antibody is able to identify Retinoic Acid Receptor alpha at its endogenous levels, but only when it is phosphorylated at Sersine 77.

Species Reactivity

Human;Mouse;Rat

Application

The recommended dilution for Western blot is 1:500 to 1:2000, while for immunohistochemistry it is 1:50 to 1:200. When it comes to ELISA with peptide, the suggested dilution is 1:20000 to 1:40000. It is important to follow the recommended dilutions to ensure accurate and reliable results in your experiments. Remember to always check the manufacturer's instructions for specific protocols and requirements for your particular assay.

Immunogen

α，77。，。

Properties

Concentration

1mg/ml

Purification

The purified rabbit serum yielded an antibody via affinity purification process using both phospho- and non-phospho-peptide affinity columns in sequence. Hence, a highly specific antibody could be obtained from the purified serum.

Clonality

Polyclonal Antibody

Stability & Storage

To ensure optimal storage conditions, it is recommended to keep the product at a temperature of -20 °C for up to one year. It is important to avoid exposing the product to repeated freeze/thaw cycles, as this can compromise the integrity of the sample. For best results, carefully follow the instructions provided by the manufacturer and handle the product with care. By storing the product at the recommended temperature and avoiding unnecessary exposure to temperature fluctuations, you can help ensure the stability and reliability of your sample.

Storage buffer

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt

Target

Background

Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. RARA plays an essential role in the regulation of retinoic acid-induced germ cell development during spermatogenesis. Has a role in the survival of early spermatocytes at the beginning prophase of meiosis. In Sertoli cells, may promote the survival and development of early meiotic prophase spermatocytes. In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function (By similarity). Regulates expression of target genes in a ligand-dependent manner by recruiting chromatin complexes containing KMT2E/MLL5. Mediates retinoic acid-induced granulopoiesis.

Posttranslational modification

Phosphorylated on serine and threonine residues. Phosphorylation does not change during cell cycle. Phosphorylation on Ser-77 is crucial for transcriptional activity (By similarity). Phosphorylation by AKT1 is required for the repressor activity but has no effect on DNA binding, protein stability nor subcellular localization. Phosphorylated by PKA in vitro. This phosphorylation on Ser-219 and Ser-369 is critical for ligand binding, nuclear localization and transcriptional activity in response to FSH signaling.Sumoylated with SUMO2, mainly on Lys-399 which is also required for SENP6 binding. On all-trans retinoic acid (ATRA) binding, a confromational change may occur that allows sumoylation on two additional site, Lys-166 and Lys-171. Probably desumoylated by SENP6. Sumoylation levels determine nuclear localization and regulate ATRA-mediated transcriptional activity.Trimethylation enhances heterodimerization with RXRA and positively modulates the transcriptional activation.Ubiquitinated.

Celluar localization

Cytoskeleton;Cytosol;Nucleus;

UniPort

P10276