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Rabbit Anti-iNOS Polyclonal Antibody #abs130136

Please note that the price mentioned above is merely for your reference, and for detailed pricing information, kindly get in touch with our sales representative, Vecent. Data Examples Western blot analysis on NIH-3T3 cell lysate using iNOS Antibody This product is for research use only, not for...

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Description

Catalog-specification	Delivery time	USD price
abs130136-50ug	1-2 Weeks	201
abs130136-100ug	1-2 Weeks	301

Please note that the price mentioned above is merely for your reference, and for detailed pricing information, kindly get in touch with our sales representative, Vecent.

Overview
Description	The iNOS enzyme plays a crucial role in producing nitric oxide (NO), which serves as a messenger molecule with a multitude of functions throughout the body. Within macrophages, NO works to promote tumoricidal and bactericidal actions. As a member of the NOS family, iNOS is actually induced by endotoxins and cytokines. Furthermore, the enzyme is stimulated by interferon gamma, which collaborates with lipopolysaccharide, tumor necrosis factor alpha, or interleukin-1 beta to increase its activity. iNOS functions as a homodimer, with a binding ability to SLC9A3R1. Notably, there are two isoforms of this protein generated by alternative splicing in human cells.
Other names	The protein known as HEP-NOS, or hepatocyte NOS, also referred to as HEPNOS, is an inducible enzyme called inducible nitric oxide synthase (iNOS) or inducible NOS. It is also known as MAC NOS or macrophage NOS. Its other names include nitric oxide synthase 2 inducible (NOS2) and nitric oxide synthase 2A (inducible, hepatocytes). This enzyme plays a crucial role in the production of nitric oxide (NO) in various cell types, including hepatocytes and macrophages. It is encoded by the NOS2 gene, also known as nos2 or NOS2_HUMAN. Additionally, HEP-NOS is associated with the activity of peptidyl-cysteine S-nitrosylase NOS2.
Source	Rabbit
Specificity	The endogenous levels of total iNOS can be detected using the iNOS antibody.
Species Reactivity	Human;Mouse;Rat
Application	Western blot (WB) is typically used at dilutions ranging from 1:500 to 1:3000. For immunohistochemistry (IHC), the usual dilution range is 1:50 to 1:200. If you're performing immunofluorescence (IF) or immunocytochemistry (ICC), the recommended dilutions are between 1:100 and 1:500. Lastly, for ELISA assays using peptides, the optimal dilution range is 1:20000 to 1:40000. These dilution ranges are based on the original information provided and can be used as a reference for your experiments.
Immunogen	A synthesized peptide derived from human iNOS.
Properties
Concentration	1mg/ml
Purification	A successful purification of the antiserum was achieved through the utilization of SulfoLink™ Coupling Resin in peptide affinity chromatography. I have rearranged the content to provide a highly similar statement, maintaining the key information of the original text. Please note that I am not generating the content in a completely different or unrelated manner.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain the integrity of the product, it is recommended to store it at a temperature of -20 °C for a duration of one year. It is important to avoid subjecting the product to freezing and thawing cycles, as this can negatively impact its quality. Ensure proper storage conditions to prolong the shelf life of the product.
Storage buffer	Phosphate-buffered saline (PBS) containing Rabbit IgG is a stable solution with a pH of 7.4. It consists of 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is recommended to store this solution at a temperature of -20 °C. The solution remains stable for a period of 12 months from the date of receipt.
Target
Background	Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body (PubMed:7531687, PubMed:7544004). In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2 (By similarity). As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM (PubMed:25417112). Involved in inflammation, enhances the synthesis of proinflammatory mediators such as IL6 and IL8 (PubMed:19688109).
Tissue specificity	Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets.
Celluar localization	Cytoskeleton;Cytosol;Nucleus;Peroxisome;
UniPort	P35228