Phospho-SHP-1 (Tyr536) Rabbit Polyclonal Antibody#abs130871

abs130871-50ug

1-2 Weeks

201

abs130871-100ug

1-2 Weeks

301

Description

The PTP family is composed of signaling molecules that play crucial roles in regulating various cellular processes, such as cell growth, differentiation, the mitotic cycle, and oncogenic transformation. This gene encodes a specific protein, which is a member of the PTP family. These proteins possess enzymatic activities that target and remove phosphate groups from tyrosine residues in proteins. This particular protein is known to be involved in modulating cellular signaling pathways and has been linked to several diseases, including cancer.

Other names

The protein tyrosine phosphatase SHP-1, also known as PTP-1C or PTPN6, is an enzyme coded by the HCPH gene. It belongs to the non-receptor type 6 of protein tyrosine phosphatases and is widely expressed in various tissues, particularly in hematopoietic cells. Another common name for SHP-1 is hematopoietic cell protein-tyrosine phosphatase.
SHP-1 plays a critical role in regulating signal transduction pathways by dephosphorylating target proteins on tyrosine residues. This activity can result in the inhibition or activation of downstream signaling events, making SHP-1 an important regulator of various biological processes such as cell proliferation, differentiation, and apoptosis.
Mutations in SHP-1 have been linked to a variety of diseases, including cancer, autoimmune disorders, and immunodeficiency. Therefore, SHP-1 represents a potential therapeutic target for the treatment of these conditions.
Given its significance, there are various aliases associated with SHP-1, including HCP, HPTP1C, SHP1L, and 70Z-SHP. Despite its many names, the function and importance of SHP-1 remain consistent across all fields of research.

Source

Rabbit

Specificity

The Phospho-SHP-1 (Tyr536) Antibody specifically identifies the presence of SHP-1 protein in its phosphorylated state at Tyrosine 536. This antibody is effective in detecting endogenous levels of phosphorylated SHP-1, providing vital information about the activation status of this protein.

Predictive reaction species

Rabbit

Application

For Western blotting, dilute the antibody at a range of 1:500 to 1:2000. For immunohistochemistry, dilute the antibody at a range of 1:50 to 1:200. For Immunofluorescence/Immunocytochemistry, dilute the antibody at a range of 1:100 to 1:500. For ELISA (peptide), dilute the antibody at a range of 1:20000-1:40000. It is important to consider these dilution ranges when using this antibody in your experiments to ensure optimal results.

A new peptide compound has been created using human SHP-1 and focusing on the Tyrosine 536 phosphorylation site. The peptide is synthetic and highly similar to the original protein. This innovation has the potential to bring significant benefits to medical research and drug development, as it provides a more cost-effective way to obtain high-quality SHP-1 without the need for expensive and time-consuming purification methods. The synthesized peptide may help researchers understand the role of SHP-1 in cellular signaling pathways and could pave the way for the development of more effective treatments for various diseases.

Properties

1mg/ml

Purification

By utilizing phospho- and non-phospho-peptide affinity columns, the antibody was extracted from purified rabbit serum through an affinity purification process. Here, the original text information is rephrased into a highly similar content.

Clonality

Polyclonal Antibody

To preserve the material, it is recommended to store it in temperatures as low as -20 °C for a period of one year. It is advised to avoid repeatedly freezing and thawing the substance, as this may have negative effects on its integrity.

Storage buffer

This product contains Rabbit IgG in phosphate buffered saline at pH 7.4, with 150mM NaCl and 0.02% sodium azide. It also contains 50% glycerol and should be stored at -20 °C. The product is stable for up to 12 months from the date of receipt.

Target

Background

 Modulates signaling by tyrosine phosphorylated cell surface receptors such as KIT and the EGF receptor/EGFR. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation. Plays a key role in hematopoiesis.

Tissue specificity

Isoform 1 is expressed in hematopoietic cells. Isoform 2 is expressed in non-hematopoietic cells.

Celluar localization

Cytosol;Extracellular region o阿r secreted;Nucleus;Plasma Membrane;

P29350