Phospho-Epo-R (Tyr368) Rabbit Polyclonal Antibody#abs130838

abs130838-50ug

1-2 Weeks

201

abs130838-100ug

1-2 Weeks

301

Other names

Source

Rabbit

Specificity

The Phospho-Epo-R (Tyr368) Antibody is specifically designed to recognize and bind to Epo-R protein levels within cells, but only when it is phosphorylated at Tyrosine 368. This antibody allows for the detection of endogenous levels of Epo-R that have undergone phosphorylation at this specific site. By selectively targeting the phosphorylated form of Epo-R, researchers can gain valuable insights into the signaling pathways and cellular processes involving this protein.

Application

The recommended dilutions for WB, IF/ICC, and ELISA(peptide) are as follows: WB dilution ranges from 1:500 to 1:2000, IF/ICC dilution ranges from 1:100 to 1:500, and ELISA(peptide) dilution ranges from 1:20000 to 1:40000. These dilutions are based on the original text information provided.

A peptide has been synthesized that is based on the human Epo-R and is designed to target the phosphorylation site of Tyrosine 368. This peptide is highly similar to the original sequence and has been created through careful rearrangement of the amino acid components. By focusing on this specific region of the Epo-R, it is hoped that this peptide can be used to better understand the interactions between human cells and erythropoietin. This peptide may also have potential therapeutic applications for conditions related to Epo-R dysfunction.

Properties

1mg/ml

Purification

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Clonality

Polyclonal Antibody

It is advisable to store at a temperature of -20 °C for a duration of one year. Make sure to prevent the occurrence of repeated freeze/thaw cycles.

Storage buffer

This product is composed of Rabbit IgG in a standardized phosphate buffered saline solution, with a pH of 7.4 and a concentration of 150mM NaCl. The solution also contains 0.02% sodium azide and 50% glycerol, and should be stored at -20 °C for optimal stability. The product is guaranteed to be stable for a period of 12 months from the date of receipt, ensuring that you have ample time to utilize it in your experiments.

Target

Background

The erythropoietin receptor (EPOR) is responsible for receiving signals from erythropoietin and promoting erythroblast proliferation and differentiation. EPOR is activated when erythropoietin binds to it, triggering the JAK2/STAT5 signaling pathway. In some instances, this receptor can also activate STAT1 and STAT3, and it may also activate the LYN tyrosine kinase. Overall, the EPOR plays a critical role in regulating erythropoiesis and maintaining normal red blood cell levels.

Tissue specificity

The abundance of erythroid cells and erythroid progenitor cells varies depending on the isoform of the erythropoietin receptor (EPOR) present. In EPO-dependent erythroleukemia cells and late-stage erythroid progenitors, isoform EPOR-F is the most prevalent form. On the other hand, isoform EPOR-S and isoform EPOR-T dominate in the bone marrow. In early-stage erythroid progenitor cells, isoform EPOR-T is the most abundant. These distinct isoforms play a crucial role in regulating the development and functioning of erythroid cells at different stages. By understanding the distribution of EPOR isoforms, we can gain insights into the specific mechanisms and pathways involved in erythropoiesis and erythroid cell differentiation. Overall, the dynamic expression of EPOR isoforms contributes to the finely tuned regulation of erythropoiesis in different cellular contexts.

Celluar localization

Extracellular region or secreted;Plasma Membrane;

P19235