Rabbit Anti-Histone H1.0 Polyclonal Antibody #abs130294

abs130294-50ug

1-2 Weeks

201

abs130294-100ug

1-2 Weeks

301

Description

Histones H1, particularly the H1F0 variant, play a crucial role in facilitating the condensation process of nucleosomes into higher order structures in cells. These histones are predominantly found in cells that have reached their final stages of differentiation or those with low cell division rates. Belonging to the histone H1/H5 family, both the unedited and RNA edited versions of H1F0 are triggered by butyrate at the protein level. However, only the RNA edited version is activated by DTT, vinblastine, or TNF at the protein level.

Other names

H1 histone family member 0, also known as H1(0) or H10, is a protein encoded by the H10_HUMAN gene. It is also referred to as h1f0, H1FV, Histone H1'', or Histone H1(0). This protein is involved in the packaging of DNA in the nucleus and is part of the histone H1 family. It is also known as Histone H1.0 or Histone H10. Another name for this protein is Histone H5. It is sometimes referred to as MGC5241 and can undergo N-terminal processing.

Source

Rabbit

Specificity

The endogenous levels of total Histone H1.0 can be detected using the Histone H1.0 antibody. This antibody is highly specific and can identify the presence of Histone H1.0 in samples. It is an effective tool for researchers who want to study the expression and function of Histone H1.0 in different biological systems. By detecting this histone protein, scientists can gain a better understanding of gene regulation and the processes that control gene expression. Overall, the Histone H1.0 antibody is a valuable resource for investigating the role of Histone H1.0 in various cellular mechanisms.

Species Reactivity

Human

Application

：(Western blot)1:500~1:3000，(IF/ICC)1:100-1:500，(ELISA)1:20000-1:40000。，。

Immunogen

Human Histone H1.0 is the source of a synthesized peptide. To create a comparable content, I will rearrange the given information. Let's ensure that the newly generated text is still in line with the original context without following the conversation pattern of ChapGPT.

Properties

Concentration

1mg/ml

Purification

To isolate the antiserum, we employed peptide affinity chromatography with the aid of SulfoLink™ Coupling Resin. This method effectively purified the antiserum, ensuring a high level of specificity and purity.

Clonality

Polyclonal Antibody

Stability & Storage

To preserve the quality of the product, it is recommended to keep it at a temperature of -20 °C for a period of one year. It is important to note that repeated freezing and thawing should be avoided as it may affect the stability and efficacy of the product.

Storage buffer

This rabbit IgG is suspended in a solution of phosphate buffered saline at pH 7.4, 150mM of NaCl, 0.02% sodium azide, and with 50% glycerol. It is recommended to store this product at a temperature of -20 °C, and it will remain stable for up to 12 months from the date of receipt.

Target

Background

Histones H1 play an important role in the compaction of nucleosome chains into more complex structures. Meanwhile, the H1F0 histones are typically present in cells that have either entered the last phase of differentiation or have lower rates of cellular division.

Tissue specificity

At the protein level, both the unedited and RNA edited versions show up as a result of exposure to butyrate. However, it is worth noting that only the RNA edited version displays induction when subjected to DTT, vinblastine, or TNF at the protein level. By rearranging the provided information, it can be conveyed that while butyrate influences both unedited and RNA edited versions at the protein level, specific inductions by DTT, vinblastine, or TNF are exclusively observed in the RNA edited version.

Posttranslational modification

Phosphorylated on Ser-17 in RNA edited version.ADP-ribosylated on Ser-104 in response to DNA damage.

Celluar localization

Cytoskeleton;Golgi apparatus;Nucleus;

UniPort

P07305