Rabbit Anti-Neuro D Polyclonal Antibody #abs130049

abs130049-50ug

1-2 Weeks

201

abs130049-100ug

1-2 Weeks

301

Description

NEUROD1 is a crucial factor involved in the development and maintenance of dendrites in the cerebellar cortex. It acts as a transcriptional activator and specifically binds to the E-box region of the insulin gene. However, for efficient DNA binding, NEUROD1 requires the formation of a dimer with another bHLH protein. It forms a heterodimer with TCF3/E47 and also interacts with RREB1. These interactions and dimerization processes play essential roles in the regulation of dendrite morphogenesis and ensure the proper functioning of the cerebellar cortex.

Other names

NeuroD1, also known as the basic helix-loop-helix transcription factor BETA2, is an important regulator of beta cell function in the pancreas. It plays a crucial role in the transcriptional control of genes involved in glucose metabolism and insulin secretion. Classified as a class A basic helix-loop-helix protein, NeuroD1 is a key player in the development and differentiation of neuronal cells.
MODY 6, or maturity onset diabetes of the young 6, is a form of diabetes that is caused by mutations in the NeuroD1 gene. This condition disrupts the normal function of beta cells in the pancreas, leading to impaired insulin production and glucose regulation.
NeuroD1, also referred to as neurogenic helix-loop-helix protein NEUROD, is involved in the process of neurogenesis, which is the formation and development of new neurons in the brain. It acts as a transcriptional regulator, controlling the expression of genes that are crucial for neuronal differentiation and maturation.
In summary, NeuroD1 is a basic helix-loop-helix transcription factor that plays multiple roles in various biological processes. Its importance in beta cell function, glucose metabolism, and neuronal development highlights its significance in both diabetes and neurobiology.

Source

Rabbit

Specificity

The Neuro D antibody has the ability to recognize and identify total Neuro D present in the body. This means that it can detect levels of the protein that are naturally present within the system.

Species Reactivity

Human;Mouse;Rat

Application

。，，1:500~1:3000。（IF）（ICC），1:100-1:500。（ELISA），1:20000-1:40000。，。

Immunogen

A synthesized peptide derived from human Neuro D.

Properties

Concentration

1mg/ml

Purification

Utilizing SulfoLink™ Coupling Resin, the purification process of the antiserum involved peptide affinity chromatography. This method efficiently separated the desired substance from unwanted contaminants, resulting in a highly purified product.

Clonality

Polyclonal Antibody

Stability & Storage

To maintain the quality of the product, it is recommended to store it at -20 °C for a year. It is important to avoid subjecting it to frequent freezing and thawing cycles.

Storage buffer

The Rabbit IgG is stored in a solution containing phosphate buffered saline at a pH of 7.4, 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is important to maintain this solution at a temperature of -20°C to prevent degradation. This Rabbit IgG solution is stable for a period of 12 months from the date of receipt.

Target

Background

Acts as a transcriptional activator: mediates transcriptional activation by binding to E box-containing promoter consensus core sequences 5'-CANNTG-3'. Associates with the p300/CBP transcription coactivator complex to stimulate transcription of the secretin gene as well as the gene encoding the cyclin-dependent kinase inhibitor CDKN1A. Contributes to the regulation of several cell differentiation pathways, like those that promote the formation of early retinal ganglion cells, inner ear sensory neurons, granule cells forming either the cerebellum or the dentate gyrus cell layer of the hippocampus, endocrine islet cells of the pancreas and enteroendocrine cells of the small intestine. Together with PAX6 or SIX3, is required for the regulation of amacrine cell fate specification. Also required for dendrite morphogenesis and maintenance in the cerebellar cortex. Associates with chromatin to enhancer regulatory elements in genes encoding key transcriptional regulators of neurogenesis (By similarity).

Posttranslational modification

Phosphorylated. In islet cells, phosphorylated on Ser-274 upon glucose stimulation; which may be required for nuclear localization. In activated neurons, phosphorylated on Ser-335; which promotes dendritic growth. Phosphorylated by MAPK1; phosphorylation regulates heterodimerization and DNA-binding activities. Phosphorylation on Ser-266 and Ser-274 increases transactivation on the insulin promoter in glucose-stimulated insulinoma cells (By similarity).

Celluar localization

Nucleus;

UniPort

Q13562