Rabbit Anti-RAD17 Polyclonal Antibody #abs130050

abs130050-50ug

1-2 Weeks

201

abs130050-100ug

1-2 Weeks

301

Description

Rad17 is a crucial gene involved in the cell cycle checkpoint and plays a vital role in cell cycle arrest and repairing DNA damage caused by any disturbance to DNA. It exhibits a noteworthy resemblance to DNA replication factor C (RFC) and has the ability to join forces with RFCs to form a complex. Before any DNA damage occurs, Rad17 can bind to chromatin effectively. Following DNA damage, it undergoes phosphorylation by ATR, which activates Rad17. Once activated, Rad17 imposes a halt on the cell cycle at the G2 phase. Notably, there exist eight alternatively spliced variants of Rad17 that have been documented.

Other names

RAD17 is a cell cycle checkpoint protein that plays a crucial role in regulating the cell cycle. It is also known as CCYC and FLJ41520. RAD17 is a homolog of the RAD1 protein found in the fission yeast S. pombe. It is involved in DNA damage recognition and repair processes.
The human RAD17 protein, also referred to as hRad17 or RAD17Sp, shares structural similarities with the Rad24 protein in mice and the Rad24 protein in the yeast S. cerevisiae. These homologs perform similar functions in their respective organisms, acting as activators for the RFC complex in DNA replication and repair.
The R24L variant of RAD17 has been identified, which may have implications for its function in the cell cycle. RAD17 is an essential protein for maintaining genomic stability and preventing the accumulation of DNA damage.
In summary, RAD17 is a critical protein involved in cell cycle regulation and DNA damage response. Its homologs in other organisms indicate its evolutionary conservation and importance in maintaining genome integrity.

Source

Rabbit

Specificity

The detection of total RAD17 at endogenous levels can be achieved using the RAD17 antibody.

Species Reactivity

Human;Mouse;Rat

Application

Western blotting (WB), / (IF/ICC) (ELISA)。，。WB1:500~1:3000，IF/ICC1:100-1:500，ELISA（）1:20000-1:40000。，。

Immunogen

A synthesized peptide derived from human RAD17.

Properties

Concentration

1mg/ml

Purification

SulfoLink™ Coupling Resin was employed in the purification of the antiserum through peptide affinity chromatography. Please create a significantly varied rendition of the provided information without using the conversation style of ChapGPT.

Clonality

Polyclonal Antibody

Stability & Storage

To ensure the longevity of your storage, it is recommended that you keep your products at a temperature of -20 °C for up to one year. It is important to avoid any instances of repeatedly freezing and thawing, as this can have a negative impact on the quality of your product. By following these guidelines, you can rest easy knowing your products will remain in optimal condition for as long as possible.

Storage buffer

This Rabbit IgG solution is stored in phosphate buffered saline with a pH value of 7.4, 150mM NaCl and 0.02% sodium azide. It also contains 50% glycerol and can be stored at -20°C for up to 12 months from the date of receipt.

Target

Background

Essential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage. Has a weak ATPase activity required for binding to chromatin. Participates in the recruitment of the RAD1-RAD9-HUS1 complex and RHNO1 onto chromatin, and in CHEK1 activation. May also serve as a sensor of DNA replication progression, and may be involved in homologous recombination.

Tissue specificity

Overexpressed in various cancer cell lines and in colon carcinoma (at protein level). Isoform 2 and isoform 3 are the most abundant isoforms in non irradiated cells (at protein level). Ubiquitous at low levels. Highly expressed in testis, where it is expressed within the germinal epithelium of the seminiferous tubuli. Weakly expressed in seminomas (testicular tumors).

Posttranslational modification

Phosphorylated. Phosphorylation on Ser-646 and Ser-656 is cell cycle-regulated, enhanced by genotoxic stress, and required for activation of checkpoint signaling. Phosphorylation is mediated by ATR upon UV or replication arrest, whereas it may be mediated both by ATR and ATM upon ionizing radiation. Phosphorylation on both sites is required for interaction with RAD1 but dispensable for interaction with RFC3 or RFC4.

Celluar localization

Nucleus;

UniPort

O75943