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Rabbit Anti-RON Polyclonal Antibody #abs130051

Description

Catalog-specification	Delivery time	USD price
abs130051-50ug	1-2 Weeks	201
abs130051-100ug	1-2 Weeks	301

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Overview
Description	Ron belongs to the Met family as a receptor tyrosine kinase. It serves as the receptor for macrophage stimulating protein. The desensitization of Ron can occur through the actions of c-Cbl and its binding partner Grb2. Interestingly, the overexpression of wild-type Ron has been linked to the development of lung tumors. Additionally, there have been two alternatively spliced isoforms of Ron described in scientific literature.
Other names	CD136, also known as c-met related tyrosine kinase, is a protein that plays an important role in various biological processes. It is also referred to as CD136 antigen or CDw136. Another name for CD136 is Macrophage Stimulating 1 Receptor (MST1R) or Macrophage Stimulating Protein Receptor. CD136 is composed of two subunits, alpha and beta chains, which together form the functional receptor. CD136 is involved in mediating cellular responses to macrophage stimulating protein (MSP). It is a transmembrane receptor that belongs to the receptor tyrosine kinase family. The MST1R gene encodes CD136, and it is located on human chromosome 3. Different isoforms and variants of CD136 have been identified, including RON30, RON62, NPCA3, p185-Ron, soluble RON variants, and RON variant E2E3. These variants may have distinct functions or characteristics. Understanding the function and regulation of CD136 is important in various biological processes, including tissue regeneration, immune response, and cancer development. CD136 signaling pathways are involved in cell proliferation, migration, and survival. In summary, CD136, also known as c-met related tyrosine kinase or MST1R, is a receptor tyrosine kinase involved in cellular responses to macrophage stimulating protein. It has various isoforms and variants with different functions, and its signaling pathways are implicated in several biological processes.
Source	Rabbit
Specificity	The RON antibody is capable of identifying the total RON protein levels within the body. This indicates that it is capable of detecting the natural or endogenous amounts of RON present in the body.
Species Reactivity	Human;Mouse
Predictive reaction species	Sheep;Bovine;Horse;Rabbit;
Application	1:500~1:3000 is the recommended dilution for Western blotting (WB), while the dilution for immunofluorescence (IF) or immunocytochemistry (ICC) is suggested to be 1:100-1:500. For ELISA using peptide antigens, the recommended dilution range is 1:20000-1:40000. It is important to note that these dilutions are based on the original information provided.
Immunogen	A synthesized peptide derived from human RON.
Properties
Concentration	1mg/ml
Purification	Using SulfoLink™ Coupling Resin, the purification of the antiserum was carried out via peptide affinity chromatography.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to MST1 ligand. Regulates many physiological processes including cell survival, migration and differentiation. Ligand binding at the cell surface induces autophosphorylation of RON on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1 or the adapter GAB1. Recruitment of these downstream effectors by RON leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. RON signaling activates the wound healing response by promoting epithelial cell migration, proliferation as well as survival at the wound site. Plays also a role in the innate immune response by regulating the migration and phagocytic activity of macrophages. Alternatively, RON can also promote signals such as cell migration and proliferation in response to growth factors other than MST1 ligand.
Tissue specificity	Expressed in colon, skin, lung and bone marrow.
Posttranslational modification	Proteolytic processing yields the two subunits.Autophosphorylated in response to ligand binding on Tyr-1238 and Tyr-1239 in the kinase domain leading to further phosphorylation of Tyr-1353 and Tyr-1360 in the C-terminal multifunctional docking site.Ubiquitinated. Ubiquitination by CBL regulates the receptor stability and activity through proteasomal degradation.
Celluar localization	Cytoskeleton;Plasma Membrane;
UniPort	Q04912