Rabbit Anti-Smad3 Antibody #abs124584

abs124584-50ul

In Stock

201

abs124584-100ul

In Stock

301

Other names

The protein known as hMAD 3, hSMAD3, or Mothers against decapentaplegic homolog 3 plays an important role in cellular signaling pathways. It is also referred to as HSPC193, JV15 2, JV152, or MAD (mothers against decapentaplegic Drosophila) homolog 3.
hSMAD3 is a member of the SMAD family of proteins, which are involved in the transforming growth factor-beta (TGF-β) signaling pathway. In particular, hSMAD3 is activated by TGF-β and transmits signals downstream to regulate gene expression and cellular function.
The function of hSMAD3 is important in many biological processes, including cell proliferation, differentiation, and migration. Mutations in hSMAD3 have been implicated in a variety of diseases, including cancer and cardiovascular disease.
Overall, understanding the role of hSMAD3 in cellular signaling pathways is important for the development of therapies to treat a variety of diseases.

Source

Rabbit

Specificity

Smad3

Species Reactivity

Human;Mouse;Rat;Chicken;Pig;Cow

Application

The recommended dilutions for various applications are as follows:
- Western Blot (WB): The suggested dilution range is 1:500 to 1:2000.
- Enzyme-Linked Immunosorbent Assay (ELISA): The appropriate dilution is 1:500 to 1:1000.
- Immunohistochemistry - Paraffin (IHC-P): Dilute the antibody at 1:400 to 1:800.
- Immunohistochemistry - Frozen (IHC-F): The ideal dilution range is 1:400 to 1:800.
- Flow Cytometry (Flow-Cyt): Use 1ug of antibody per test.
- Immunocytochemistry (ICC): The recommended dilution is 1:100 to 1:500.
- Immunofluorescence (IF): Dilute the antibody at 1:100 to 1:500. Please note that antigen retrieval is necessary for paraffin sections.

Immunogen

A synthetic peptide derived from human Smad3 (31-80aa) conjugated with KLH is available for purchase at position 31-80/425. You can obtain a similar content by rearranging the information provided above while ensuring that it stays true to the original text.

Properties

Concentration

1mg/ml

Purification

affinity purified by Protein A

Clonality

Polyclonal Antibody

Isotype

IgG

Stability & Storage

To ensure the stability of the product, it is recommended to store it at a temperature of -20 °C for a period of one year. It is important to avoid subjecting it to repeated freeze/thaw cycles, as this can have an adverse effect on its quality. Therefore, it is crucial to follow the recommended storage conditions to maintain the integrity and effectiveness of the product.

Storage buffer

0.01M TBS(pH7.4)1%（BSA），0.03% Proclin30050%。，。

Research area

The field of cell biology encompasses a range of topics, including the study of tumors, immunology, signal transduction, and stem cells. A key focus of research in this field is the process of apoptosis, or programmed cell death, which plays a critical role in maintaining healthy tissues and preventing the growth of cancerous cells.
Other important areas of study in cell biology include the role of growth factors and hormones, which regulate cell division and differentiation. Transcription regulators are also of great interest to researchers in this field, as they play a critical role in controlling gene expression and ultimately determining the behavior of cells within the body.
Overall, the study of cell biology is essential for understanding the fundamental processes that underlie the development and functioning of living organisms. By investigating the molecular mechanisms that govern cellular processes, researchers are working to develop new therapies for a wide range of diseases, from cancer to autoimmune disorders.

Target

Background

Smad3, a 50 kDa protein, belongs to a family of proteins that play a vital role as key mediators in cell proliferation, differentiation, and development through TGF-beta superfamily signaling. The Smad family consists of three subclasses: receptor regulated Smads, activin/TGF-beta receptor regulated (Smad2 and 3), or BMP receptor regulated (Smad 1, 5, and 8). Additionally, there is a common partner, Smad4, which interacts with various Smads, and inhibitory Smads, namely Smad6 and 7. Upon TGF-beta stimulation, Smad3 becomes activated, forms oligomers with Smad4, and together they translocate into the nucleus as a complex. This translocation allows their binding to DNA and transcription factors. The phosphorylation of serines 423 and 425 in the C terminus of Smad3, which is dependent on TGF-beta, is of utmost importance for Smad3's transcriptional activity and its role in TGF-beta signaling. Feel free to rearrange this information to generate new content.

Celluar localization

Nucleus cytoplasm

UniPort

Q15796