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Rabbit Anti-PODN Polyclonal Antibody#abs139688

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Description

Catalog-specification	Delivery time	USD price
abs139688-100ug	1-2 Weeks	301.0
abs139688-50ug	1-2 Weeks	201.0

Overview
catalog	abs139688
Other names	MGC24995 (also known as Podocan proteoglycan) is a protein encoded by the gene PODN_HUMAN, also referred to as Podocan. It is located on chromosome 18 and is specifically associated with the gene RP23 40G2.7. PCAN is an abbreviation commonly used to represent this protein. Another name for this gene is SLRR5A.
Source	Rabbit
Specificity	The levels of total PODN can be detected by the PODN Antibody, which specifically targets the endogenous levels of this protein. To create a highly similar content, the rearranged version can be: The endogenous levels of total PODN can be accurately measured using the PODN Antibody. This antibody specifically recognizes PODN and allows for the detection of its total levels in biological samples.
Species Reactivity	Human
Predictive reaction species	Sheep;Bovine;Dog;Horse;
Antigen	PODN
Application	WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000
Immunogen	A synthesized peptide derived from human PODN.
MW	69 kDa
Properties
Concentration	1mg/ml
purification	In order to purify the antiserum, peptide affinity chromatography was employed and SulfoLink™ Coupling Resin was used for this process. The chromatography column was packed with the resin, which was covalently linked to peptides specific to the target antigen. The antiserum was then applied to the column, and the antibodies that specifically bound to the peptide epitopes were retained, while the non-specific antibodies were eluted. The purified antiserum was collected from the column and analyzed for purity and activity. Overall, this method was highly effective in producing a pure and potent antiserum for further use in biological assays and research.
Clonality	Polyclonal Antibody
Stability & Storage	To ensure long-term preservation, it is recommended to store at a temperature of -20 °C for a duration of one year. It is advised to avoid subjecting the stored items to repeated cycles of freezing and thawing.
Storage buffer	The storage conditions for Rabbit IgG are as follows: it should be stored at -20 °C in phosphate buffered saline with a pH of 7.4. The solution should also contain 150mM NaCl, 0.02% sodium azide, and 50% glycerol. This IgG is stable for a period of 12 months from the date of receipt.
Target
Background	Negatively regulates cell proliferation and cell migration.
Tissue specificity	Kidney, heart, liver, pancreas and vascular smooth muscle cells. Also detected in aortic intima (at protein level).
Posttranslational modification	N-glycosylated.
Celluar localization	Extracellular region or secreted;
UniPort	Q7Z5L7