Rabbit Anti-THRB Polyclonal Antibody #abs130292

abs130292-50ug

1-2 Weeks

201

abs130292-100ug

1-2 Weeks

301

Description

Thrombin is a crucial enzyme in blood coagulation that plays a significant role in maintaining homeostasis, wound healing, and the inflammatory response. It belongs to the peptidase S1 family and functions by cleaving bonds after Arg and Lys residues. Among its numerous activities, the most important are the conversion of fibrinogen to fibrin and the activation of clotting factors V, VII, VIII, XIII. Further, when paired with thrombomodulin, thrombin can activate a natural anticoagulant, protein C.

Other names

Thrombin, also known as coagulation factor II or Factor II, is a serine protease that plays a crucial role in the blood clotting process. It is produced as a prepro coagulation factor II in the liver and is cleaved to form prothrombin, which consists of a heavy chain and a light chain. Prothrombin is activated to thrombin by cleavage of the light chain, exposing the active site.
Thrombin is involved in several pathways in blood coagulation. It cleaves fibrinogen to form fibrin, which polymerizes to form a stable blood clot. Thrombin also activates other coagulation factors, such as Factor V and Factor VII, in a positive feedback loop, leading to further clot formation.
Thrombin is encoded by the F2 gene and mutations in this gene can lead to various bleeding disorders. The PT test, or prothrombin time, is commonly used to monitor the blood clotting process in patients on anticoagulant therapy.
Overall, thrombin is a critical enzyme in the blood clotting process and understanding its function and regulation is crucial for managing bleeding disorders and preventing thrombotic events.

Source

Rabbit

Specificity

The THRB antibody has the ability to identify total levels of endogenous THRB protein.

Species Reactivity

Human;Mouse;Rat

Application

For Western blotting, the recommended dilution range for this antibody is 1:500 to 1:3000. When using it for immunohistochemistry, a dilution of 1:50 to 1:200 is suggested. For immunofluorescence and immunocytochemistry, the antibody should be diluted in the range of 1:100 to 1:500. In ELISA utilizing a peptide substrate, the antibody should be diluted to a range of 1:20000-1:40000. It is important to note that these dilution ranges are only recommendations, and the optimal dilution may vary based on the specific experiment conditions.

Immunogen

A synthesized peptide derived from human THRB

Properties

Concentration

1mg/ml

Purification

Affinity-chromatography

Clonality

Polyclonal Antibody

Stability & Storage

To maintain the integrity of the product, it is recommended to store it at a temperature of -20 °C for a period of one year. It is important to avoid repetitive freezing and thawing cycles to ensure optimal performance and quality. By following these guidelines, you can ensure that the product remains stable and effective for its intended use over an extended period of time.

Storage buffer

The storage conditions for Rabbit IgG are as follows: it should be stored at -20 °C. It is recommended to store it in phosphate buffered saline with a pH of 7.4, containing 150mM NaCl, 0.02% sodium azide, and 50% glycerol. The stability of the IgG is guaranteed for 12 months from the date of receipt.

Target

Background

Thrombin plays a crucial role in various physiological processes such as blood clotting regulation, wound healing, and inflammation. This multifunctional enzyme selectively cleaves bonds located after Arginine (Arg) and Lysine (Lys) residues. By doing so, it converts fibrinogen, a soluble plasma protein, into insoluble fibrin, which forms the framework of blood clots. Additionally, thrombin also acts as an activator for several factors involved in blood clotting, namely factors V, VII, VIII, and XIII.
Moreover, when thrombin forms a complex with thrombomodulin, it exerts anticoagulant effects by activating protein C, which plays a crucial role in regulating coagulation and preventing excessive clot formation. Thus, thrombin contributes to maintaining the delicate balance of blood homeostasis. Furthermore, beyond its role in blood clotting, thrombin also participates in inflammatory processes and wound healing, highlighting its significance in various physiological and pathological contexts.

Tissue specificity

Expressed by the liver and secreted in plasma.

Posttranslational modification

The formation of gamma-carboxyglutamyl residues is facilitated by the vitamin K-dependent carboxylase enzyme, which converts glutamyl residues by carboxylation and creates a binding site for calcium ions. This binding site is crucial for the interaction of prothrombin with a negatively charged phospholipid surface, which is required for its conversion into thrombin. The protein is N-glycosylated at two sites - Asn-121 and Asn-143 - with different levels of heterogeneity. At Asn-121, the N-glycan has three hexose units, with minor variation in the exact arrangement of the sugar residues. At Asn-143, the major N-glycan has four hexose units and four hexosamine units, while a minor population has three hexose units and three hexosamine units. These modifications contribute to the stability and functionality of the protein in the coagulation pathway.

Celluar localization

Endoplasmic reticulum;Extracellular region or secreted;Golgi apparatus;Plasma Membrane;

UniPort

P00734