Rabbit Anti-MYC Polyclonal Antibody #abs130293

abs130293-50ug

1-2 Weeks

201

abs130293-100ug

1-2 Weeks

301

Description

Myc, an oncogenic transcription factor, is crucial in the regulation of cell proliferation, apoptosis, and the formation of human tumors. It shows a propensity for stimulating the transcription of genes that are closely associated with growth.

Other names

Myc proto-oncogene protein, also known as Avian myelocytomatosis viral oncogene homolog (MYC), is a transcription factor belonging to the Class E basic helix-loop-helix protein 39 (bHLHe39) family. MYC plays a crucial role in cell proliferation, differentiation, and apoptosis. It is implicated in various cancers, including myelocytomatosis oncogene and Nird. MYC is also known as Proto-oncogene c-Myc, V-Myc avian myelocytomatosis viral oncogene homolog, and Transcription factor p64.
Additionally, MYC has been associated with myc-related translation/localization regulatory factor (MRTL) and can act as a transcriptional regulator through its isoform Myc-A. Other aliases of MYC include Myc2, MYCC, Niard, RNCMYC, and Oncogene Myc. The gene encoding MYC is labeled as OTTHUMP00000158589 and is homologous to the myelocytomatosis virus protooncogene.
To summarize, Avian myelocytomatosis viral oncogene homolog, or MYC, is a well-known transcription factor involved in oncogenesis, cell regulation, and cancer development. Its diverse aliases and isoforms reflect its significance and wide-ranging functions in cellular processes.

Source

Rabbit

Specificity

Detection of endogenous levels of total MYC can be accomplished using the MYC antibody. The antibody is specifically designed to recognize and bind to the total MYC protein in biological samples. By employing this antibody, researchers can investigate the presence and quantity of MYC in various tissues or cell types. The MYC antibody is a valuable tool for studying the expression and regulation of MYC, which plays a vital role in various cellular processes.

Species Reactivity

Human;Mouse;Rat

Application

To ensure accurate results, it is recommended to dilute the antibody at different ratios for various applications. For Western blotting, a dilution of 1:500 to 1:3000 is recommended, while for immunohistochemistry, a dilution of 1:50 to 1:200 is ideal. For fluorescence-based techniques such as IF/ICC, a dilution of 1:100 to 1:500 is optimal. When using the antibody for ELISA using a peptide as the target antigen, a dilution of 1:20000 to 1:40000 is advised. It is crucial to determine the correct antibody dilution for specific applications to obtain reliable and reproducible results.

Immunogen

A synthesized peptide derived from human MYC.

Properties

Concentration

1mg/ml

Purification

SulfoLink™ Coupling Resin was utilized in the peptide affinity chromatography to purify the antiserum.

Clonality

Polyclonal Antibody

Stability & Storage

To maintain its quality, it is essential to store the item at a temperature of -20 °C for a duration of one year. It is important to avoid subjecting it to repeated freeze/thaw cycles.

Storage buffer

Phosphate buffered saline containing Rabbit IgG, with a pH of 7.4, 150mM NaCl, 0.02% sodium azide, and 50% glycerol, should be stored at a temperature of -20 °C. This product is stable for a duration of 12 months starting from the date of purchase.

Target

Background

This transcription factor is capable of binding DNA in a non-specific matter, yet it also possesses specific recognition abilities for the core sequence 5'-CAC[GA]TG-3'. Its activation leads to the promotion of growth-related genes transcription. Additionally, it plays a key role in binding to the VEGFA promoter, which in turn results in the enhancement of VEGFA production that leads to sprouting angiogenesis (as found in PubMed:24940000).

Posttranslational modification

Phosphorylated by PRKDC. Phosphorylation at Ser-329 by PIM2 leads to the stabilization of MYC (By similarity). Phosphorylation at Ser-62 by CDK2 prevents Ras-induced senescence. Phosphorylated at Ser-62 by DYRK2; this primes the protein for subsequent phosphorylation by GSK3B at Thr-58. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome.Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28, due to the lack of interaction between isoform 4 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRUSS) complex. Ubiquitinated by TRIM6 in a phosphorylation-independent manner (By similarity).

Celluar localization

Cytosol;Nucleus;

UniPort

P01106