Phospho-eNOS(Ser615) Rabbit Polyclonal Antibody #abs130011

abs130011-50ug

1-2 Weeks

201

abs130011-100ug

1-2 Weeks

301

Description

Endothelial constitutive nitric oxide synthase, known as eNOS, is a critical enzyme within the body that facilitates the creation of nitric oxide (NO) from arginine and oxygen. NO plays a key role in smooth muscle relaxation within the vascular system through the mediation of cGMP-mediated signal transduction pathways. Additionally, eNOS has been linked to the promotion of angiogenesis in coronary vessels through the activity of vascular endothelial growth factor (VEGF). However, it is important to note that NO can also participate in blood clotting by activating platelets, indicating a complex relationship between eNOS and the cardiovascular system.

Other names

There are many names for the enzyme nitric oxide synthase 3 (NOS3) that is produced in endothelial cells. These include eNOS, Constitutive NOS, EC-NOS, ecNOS, Endothelial nitric oxide synthase, and Endothelial nitric oxide synthase 3. All of these names refer to the same enzyme that is responsible for producing nitric oxide in the endothelium. Nitric oxide is important for regulating blood flow and vascular tone in the body. Additionally, NOS3 plays a role in other physiological processes such as angiogenesis and platelet function. Overall, despite the different names, the enzyme NOS3 has a crucial role in maintaining the health of the cardiovascular system.

Source

Rabbit

Specificity

The Phospho-eNOS(Ser615) Antibody specifically recognizes eNOS which is phosphorylated at Serine 615 and can detect endogenous levels of the protein. The antibody does not detect non-phosphorylated eNOS. This information indicates the specificity of the antibody for a phosphorylation site on eNOS and its usefulness in studying this specific modification of the protein.

Species Reactivity

Human;Mouse;Rat

Application

The recommended dilution for Western blotting (WB) ranges from 1:500 to 1:2000. For immunofluorescence/immunocytochemistry (IF/ICC), the suggested dilution is between 1:100 and 1:500. When it comes to ELISA using a peptide, it is advised to dilute the sample from 1:20000 to 1:40000. These dilution ratios ensure optimal experimental conditions for each technique.

Immunogen

eNOS615，，。

Properties

Concentration

1mg/ml

Purification

The affinity-purified antibody was obtained by subjecting purified rabbit serum to sequential chromatography on affinity columns containing phospho- and non-phospho-peptides. Please note that this generated content is based on the original information provided, but presented in a different format.

Clonality

Polyclonal Antibody

Stability & Storage

Make sure to keep the store at a temperature of -20 °C for a duration of one year. It is important to prevent any repeated freeze/thaw cycles during this time.

Storage buffer

The storage solution for Rabbit IgG comprises phosphate buffered saline at pH 7.4, with 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It should be kept at -20°C and is known to maintain its stability for up to 12 months from the date of receipt.

Target

Background

Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.

Tissue specificity

Platelets, placenta, liver and kidney.

Posttranslational modification

Phosphorylation by AMPK at Ser-1177 in the presence of Ca2+-calmodulin (CaM) activates activity. In absence of Ca2+-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity (By similarity). Phosphorylation of Ser-114 by CDK5 reduces activity.

Celluar localization

Cytoskeleton;Cytosol;Golgi apparatus;Nucleus;Plasma Membrane;

UniPort

P29474