Phospho-HBP1(Ser402) Rabbit Polyclonal Antibody #abs130009

abs130009-50ug

1-2 Weeks

201

abs130009-100ug

1-2 Weeks

301

Description

HBP1 trembl

Other names

High mobility group box transcription factor 1, also known as HBP 1 or HBP1, is a protein that contains an HMG box and is involved in transcriptional regulation. It is encoded by the HBP1_HUMAN gene and is also referred to as HMG box-containing protein 1 or HMG box transcription factor 1. HBP1 plays a critical role in gene expression and is implicated in various cellular processes, including cell proliferation and differentiation. Furthermore, it has been identified as a potential tumor suppressor gene, and alterations in HBP1 expression have been observed in multiple cancer types. HBP1 is a key regulator of several important pathways, making it a promising target for therapeutic interventions.

Source

Rabbit

Specificity

The antibody for Phospho-HBP1(Ser402) specifically recognizes phosphorylated HBP1 at Serine 402, allowing for the detection of endogenous levels of this protein. To ensure accuracy, it is important to rearrange the content while maintaining the original information. So here it is:
Detection of HBP1 requires the presence of phosphorylation at Serine 402. The Phospho-HBP1(Ser402) Antibody is designed to specifically detect this phosphorylated form of HBP1, enabling identification of endogenous levels of the protein.

Species Reactivity

Human

Application

The recommended dilutions for WB are 1:500-1:2000, while for IF/ICC they are 1:100-1:500. For ELISA using peptide samples, the suggested dilutions are 1:20000-1:40000. These dilutions will ensure effective and accurate results when using these techniques for protein detection and analysis. It is important to follow these recommendations to achieve the desired outcomes and to avoid any potential errors or inaccuracies in the data. When conducting experiments, it is always advisable to optimize the dilution factor according to the specific conditions and requirements of the experiment.

Immunogen

An artificial peptide was created using human HBP1 as a template, focusing on the phosphorylation area surrounding Serine 402. To produce a comparable interpretation, the phrasing and terminology of the initial text were retained.

Properties

Concentration

1mg/ml

Purification

The purified rabbit serum was used to obtain the antibody through affinity purification. This process involved sequential chromatography on both phospho- and non-phospho-peptide affinity columns. The resulting antibody is a highly specific and efficient tool for use in various applications. Its production methodology ensures consistent and reliable performance for reliable and accurate results every time.

Clonality

Polyclonal Antibody

Stability & Storage

To ensure the preservation of the product, it is recommended to store it at a temperature of -20 °C for a duration of one year. It is important to avoid subjecting the product to repeated freeze/thaw cycles.

Storage buffer

You can store Rabbit IgG in phosphate-buffered saline at pH 7.4 with 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is recommended to store it at -20 °C. This product remains stable for 12 months from the date of receipt.

Target

Background

A transcriptional repressor has the ability to bind to specific promoter regions of target genes, ultimately regulating the cell cycle and the Wnt pathway. Its binding affinity is predominantly targeted towards the sequence 5'-TTCATTCATTCA-3'. Supporting this affinity, the interaction between DNA and TCF4 is disrupted. Interestingly, RB1 enhances the binding of this repressor to the H1F0 promoter.

Celluar localization

Nucleus;

UniPort

O60381