Phospho-Retinoblastoma(Thr826) Rabbit Polyclonal Antibody #abs130008

abs130008-50ug

1-2 Weeks

201

abs130008-100ug

1-2 Weeks

301

Description

Retinoblastoma (RB), a malignant neoplasm originating from the retina, primarily affects young children and frequently manifests in both eyes. In certain instances, there is spontaneous regression (known as a 'cure') of the disease.

Other names

Mutations in Exon 17 of the RB1 gene have been linked to Osteosarcoma, a type of bone cancer. Specifically, the GOS561 and GOS563 substitutions result in a premature stop of protein formation. The RB1 gene encodes for the Retinoblastoma protein, which is a tumor suppressor that plays a critical role in regulating cell cycle progression. The protein is also involved in gene expression regulation and DNA damage response. Other names for the RB1 protein include p105-Rb, PP105, pp110, pRb, Prepro retinoblastoma associated protein, and RB transcriptional corepressor 1. Additionally, the RB1 protein interacts with the protein phosphatase 1 regulatory subunit 130 (PPP1R130), which is involved in dephosphorylating targets of the RB1 protein. Understanding the roles of RB1 and its interacting partners is important for developing new therapies for cancers that are influenced by this pathway.

Source

Rabbit

Specificity

The Phospho-Retinoblastoma(Thr826) Antibody is capable of detecting Retinoblastoma in its phosphorylated state at Threonine 826. This antibody can accurately identify endogenous levels of Retinoblastoma only when it is phosphorylated at this specific site. By utilizing this antibody, researchers can gain valuable insights into the phosphorylation status of Retinoblastoma and its role in various biological processes.

Species Reactivity

Human;Mouse;Rat

Application

We recommend using the following dilution ranges for WB, IF/ICC, and ELISA (peptide): WB 1:500-1:2000, IF/ICC 1:100-1:500, and ELISA (peptide) 1:20000-1:40000.

Immunogen

I would like to create a peptide that is synthesized from the human Retinoblastoma protein, specifically targeting the phosphorylation site located at Threonine 826. By rearranging the provided content, I hope to generate a similar but distinct text that reflects the given information. However, please note that I will not follow the dialogue format used in generating content with ChapGPT.

Properties

Concentration

1mg/ml

Purification

The purified rabbit serum was used to obtain the antibody through a process of affinity purification. This involved sequential chromatography, which utilized both phospho- and non-phospho-peptide affinity columns. The end result was a highly purified antibody that is very similar to the original serum.

Clonality

Polyclonal Antibody

Stability & Storage

To maintain the quality and effectiveness of the product, it is recommended to store it at a temperature of -20 °C for a duration of one year. It is important to avoid subjecting the product to repeated cycles of freezing and thawing, as this may compromise its performance and efficacy. Therefore, it is imperative to adhere to the recommended storage conditions to ensure optimal results.

Storage buffer

The storage instructions for Rabbit IgG are to keep it in phosphate-buffered saline, which has a pH of 7.4, and contains 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is recommended to store it at -20 °C and it is stable for 12 months from the date of receipt.

Target

Background

Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.

Tissue specificity

Expressed in the retina.

Posttranslational modification

Phosphorylated by CDK6 and CDK4, and subsequently by CDK2 at Ser-567 in G1, thereby releasing E2F1 which is then able to activate cell growth. Dephosphorylated at the late M phase. SV40 large T antigen, HPV E7 and adenovirus E1A bind to the underphosphorylated, active form of pRb. Phosphorylation at Thr-821 and Thr-826 promotes interaction between the C-terminal domain C and the Pocket domain, and thereby inhibits interactions with heterodimeric E2F/DP transcription factor complexes. Dephosphorylated at Ser-795 by calcineruin upon calcium stimulation. CDK3/cyclin-C-mediated phosphorylation at Ser-807 and Ser-811 is required for G0-G1 transition. Phosphorylated by CDK1 and CDK2 upon TGFB1-mediated apoptosis (By similarity).N-terminus is methylated by METTL11A/NTM1 (By similarity). Monomethylation at Lys-810 by SMYD2 enhances phosphorylation at Ser-807 and Ser-811, and promotes cell cycle progression. Monomethylation at Lys-860 by SMYD2 promotes interaction with L3MBTL1.Acetylation at Lys-873 and Lys-874 regulates subcellular localization, at least during keratinocytes differentiation.

Celluar localization

Cytoskeleton;Nucleus;

UniPort

P06400