Phospho-ERK8(Thr175+Tyr177) Rabbit Polyclonal Antibody #abs130007

abs130007-50ug

1-2 Weeks

201

abs130007-100ug

1-2 Weeks

301

Description

In vitro studies have shown that ERK7 phosphorylates MBP. The protein also has interactions with CSK/c-Src, ABL1 and RET. Humans have three isoforms of ERK7 that are generated by alternative splicing.

Other names

Extracellular signal-regulated kinase 7, also known as ERK-7 or MAP kinase 15, is an important protein involved in intracellular signaling pathways. It is part of the mitogen-activated protein kinase (MAPK) family and plays a role in cell growth, differentiation, and survival.
ERK-7, also referred to as Extracellular regulated kinase 8 delta, has a close homolog known as ERK-8 or Extracellular signal-regulated kinase 8. These two proteins share similar characteristics and functions in cellular signaling processes.
Furthermore, ERK7 and ERK8 are interchangeably referred to as MAPK 15 or Mitogen-activated protein kinase 15. This name highlights their involvement in the regulation of cellular responses to external stimuli.
Overall, ERK-7 and ERK-8, or MAPK 15, are key players in the complex network of signaling pathways that control various cellular activities. Their activation and regulation contribute to important physiological processes, including cell proliferation, differentiation, and survival. Understanding their roles and interactions can provide valuable insights into the mechanisms underlying normal and pathological conditions.

Source

Rabbit

Specificity

ERK8 can be detected using the Phospho-ERK8(Thr175+Tyr177) Antibody, specifically targeting the phosphorylation of Threonine 175 and Tyrosine 177 residues. This antibody is designed to recognize endogenous levels of ERK8 only when it is phosphorylated at these specific sites.

Species Reactivity

Human;Mouse

Application

The recommended dilutions for Western blot are between 1:500 and 1:2000, while the dilutions for immunofluorescence or immunocytochemistry are between 1:100 and 1:500. For ELISA using peptides, the suggested dilutions range from 1:20000 to 1:40000. It's important to follow these recommended dilutions to ensure accurate and reliable results in your experiments.

Immunogen

A newly synthesized peptide has been developed using human ERK8 and encompassing the phosphorylation site of Threonine 175 and Tyrosine 177. This peptide is highly similar to the original amino acid sequence and is expected to have notable biological activity. By utilizing modern scientific techniques, this peptide has the potential to be a valuable tool in the study of cellular signaling pathways. Its development represents a significant advancement in our understanding of ERK8 signaling and presents exciting new opportunities for future research endeavors.

Properties

Concentration

1mg/ml

Purification

The purified rabbit serum was utilized to obtain the antibody through affinity purification. This was done by sequentially subjecting it to chromatography on phospho- and non-phospho-peptide affinity columns. As a result, a highly specific and targeted antibody was obtained, which can be used in various applications.

Clonality

Polyclonal Antibody

Stability & Storage

To preserve the product's quality, it is recommended to store it for one year at -20 °C. It is important to avoid subjecting it to repeated freeze/thaw cycles.

Storage buffer

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To ensure stability and preserve its quality, the Rabbit IgG is stored at -20 °C in a solution containing phosphate buffered saline at pH 7.4, 150mM NaCl, 0.02% sodium azide, and 50% glycerol. This storage condition allows the Rabbit IgG to remain stable for a period of 12 months from the date of receipt.

Target

Background

In vitro, phosphorylates MBP.

Tissue specificity

Widely expressed with a maximal expression in lung and kidney.

Posttranslational modification

Dually phosphorylated on Thr-175 and Tyr-177, which activates the enzyme. Autophosphorylated on threonine and tyrosine residues in vitro.Ubiquitinated. Ubiquitination may allow its tight kinase activity regulation and rapid turnover. May be ubiquitinated by a SCF E3 ligase (By similarity).

Celluar localization

Extracellular region or secreted;Nucleus;

UniPort

Q8TD08