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Rabbit Anti-EHMT1 Polyclonal Antibody#abs132870

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Description

Catalog-specification	Delivery time	USD price
abs132870-100ug	1-2 Weeks	301.0
abs132870-50ug	1-2 Weeks	201.0

Overview
catalog	abs132870
Description	This protein is a histone methyltransferase that specifically targets the lysine 9 residue of histone H3 (H3K9). It is responsible for adding one or two methyl groups (mono- and dimethylation) to this residue in regions of euchromatin. The addition of these methyl groups, known as H3K9me1 and H3K9me2, respectively, serves as an epigenetic mark for transcriptional repression. This mark recruits proteins called HP1, which bind to the methylated histones and contribute to gene silencing. In addition to its role in H3K9 methylation, this protein also weakly methylates the lysine 27 residue of histone H3 (H3K27me). It is involved in DNA methylation, but interestingly, its histone methyltransferase activity is not required for this function. This suggests that the two activities, DNA methylation and histone methylation, operate independently. The protein is likely guided to histone H3 by various DNA-binding proteins such as E2F6, MGA, MAX, and/or DP1. During the G0 phase of the cell cycle, it is believed to contribute to the repression of MYC- and E2F-responsive genes, potentially playing a role in the transition from G0 to G1. Beyond its involvement in histone methylation, this protein also methylates proteins other than histones. One of its targets is the tumor suppressor protein p53/TP53, specifically at the lysine 373 residue, where it adds two methyl groups (dimethylation).
Other names	EHMT1, also known as Eu-HMTase1 or Euchromatic histone lysine N-methyltransferase 1, is a protein encoded by the BA188C12.1 and DKFZp667M072 genes in humans. It is responsible for methylating histone H3 at lysine 9, thereby regulating gene expression and chromatin remodeling. EHMT1 is also sometimes referred to as G9a-like protein 1 or GLP1 due to its close similarity to the G9a histone methyltransferase. Mutations in EHMT1 and its dysregulation have been linked to various human diseases and disorders including schizophrenia, intellectual disability, and cancer. This protein is highly important for maintaining proper cellular function and growth, making it a topic of intense research in both basic and clinical sciences.
Source	Rabbit
Specificity	The EHMT1 antibody has the ability to identify the complete EHMT1 present in the body. This means it is able to pick up on the natural levels of EHMT1 without any artificial intervention.
Species Reactivity	Human;Mouse
Predictive reaction species	Pig;Rabbit;Sheep;Dog;Bovine;Horse;
Antigen	EHMT1
Application	WB 1:500-1:2000, ELISA(peptide) 1:20000-1:40000
Immunogen	A synthesized peptide.
MW	170 kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Target
Background	Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. During G0 phase, it probably contributes to silencing of MYC- and E2F-responsive genes, suggesting a role in G0/G1 transition in cell cycle. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53.
Tissue specificity	Widely expressed.
Celluar localization	Nucleus;
UniPort	Q9H9B1