Phospho-ALK (Tyr1507) Rabbit Polyclonal Antibody#abs131114

abs131114-50ug

1-2 Weeks

201

abs131114-100ug

1-2 Weeks

301

Description

ALK is a member of the tyrosine kinase family that plays a crucial role in brain development and affects specific neurons in the nervous system. Its expression as a fusion protein is observed in anaplastic lymphoma. This protein is translocated and has significant implications in the development of the brain and its impact on neuronal activity in the nervous system.

Other names

ALK is a receptor called ALK tyrosine kinase receptor. It is associated with two fusion genes: ALK/EML4 fusion gene and ALK/NPM1 fusion gene. ALK_HUMAN is the gene symbol for the ALK gene. It is also known as anaplastic lymphoma kinase (Ki-1), Anaplastic lymphoma kinase, Anaplastic lymphoma kinase Ki1, anaplastic lymphoma receptor tyrosine kinase, CD 246, CD246, CD246 antigen, EC 2.7.10.1, Ki 1, Ki1, mutant anaplastic lymphoma kinase, NBLST 3, NBLST3, Tcrz, and TFG/ALK.

Source

Rabbit

Specificity

The Phospho-ALK (Tyr1507) Antibody is specifically designed to recognize the presence of phosphorylated ALK at Tyrosine 1507. This antibody is sensitive enough to detect endogenous levels of ALK protein only when it is phosphorylated at this specific site.

Predictive reaction species

Zebrafish;Rabbit;Pig;Dog;Bovine

Application

For Western blotting, a dilution of 1:500 to 1:2000 is recommended, while for immunohistochemistry a dilution of 1:50 to 1:200 is advised. If using immunofluorescence or immunocytochemistry, the recommended dilution is 1:100 to 1:500. For ELISA using a peptide substrate, a dilution of 1:20000 to 1:40000 is recommended. These dilution values should serve as a guideline for optimal results, but may vary depending on the specific experiment being performed.

A peptide synthesized from the region of human ALK encompassing the phosphorylation site located at Tyrosine 1507 has been developed. The peptide closely resembles the original sequence and can be utilized for various research purposes related to ALK biology.

Properties

1mg/ml

Purification

To obtain the antibody, rabbit serum was purified and subjected to sequential chromatography using both phospho- and non-phospho-peptide affinity columns. The resulting product was highly purified and specific to the target antigen. This purification process ensured the removal of any unwanted contaminants and resulted in a high-quality antibody suitable for use in various applications. Ultimately, this method allowed for the successful production of a superior antibody that can reliably and accurately detect the target protein.

Clonality

Polyclonal Antibody

To maintain the quality of the product, it is recommended to store it at a temperature of -20 °C for a duration of one year. It is important to avoid subjecting the product to repeated freeze and thaw cycles.

Storage buffer

This solution contains Rabbit IgG in a buffered saline solution with a pH of 7.4, 150mM of NaCl, 0.02% sodium azide, and 50% glycerol. It's recommended to store this solution at a temperature of -20°C and it's stable for up to 12 months from the date of receipt.

Target

Background

 Neuronal receptor tyrosine kinase that is essentially and transiently expressed in specific regions of the central and peripheral nervous systems and plays an important role in the genesis and differentiation of the nervous system. Transduces signals from ligands at the cell surface, through specific activation of the mitogen-activated protein kinase (MAPK) pathway. Phosphorylates almost exclusively at the first tyrosine of the Y-x-x-x-Y-Y motif. Following activation by ligand, ALK induces tyrosine phosphorylation of CBL, FRS2, IRS1 and SHC1, as well as of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Acts as a receptor for ligands pleiotrophin (PTN), a secreted growth factor, and midkine (MDK), a PTN-related factor, thus participating in PTN and MDK signal transduction. PTN-binding induces MAPK pathway activation, which is important for the anti-apoptotic signaling of PTN and regulation of cell proliferation. MDK-binding induces phosphorylation of the ALK target insulin receptor substrate (IRS1), activates mitogen-activated protein kinases (MAPKs) and PI3-kinase, resulting also in cell proliferation induction. Drives NF-kappa-B activation, probably through IRS1 and the activation of the AKT serine/threonine kinase. Recruitment of IRS1 to activated ALK and the activation of NF-kappa-B are essential for the autocrine growth and survival signaling of MDK.

Tissue specificity

Expressed in brain and CNS. Also expressed in the small intestine and testis, but not in normal lymphoid cells.

Celluar localization

Extracellular region or secreted;Plasma Membrane;

Q9UM73