Phospho-DARPP-32 (Thr75) Rabbit Polyclonal Antibody#abs131112

abs131112-50ug

1-2 Weeks

201

abs131112-100ug

1-2 Weeks

301

Description

DARPP-32 is a member of the protein phosphatase inhibitor 1 family, functioning as a dopamine- and cyclic AMP-regulated neuronal phosphoprotein. The phosphorylation status of DARPP32 is modulated by both dopaminergic and glutamatergic (NMDA) receptor stimulation, but interestingly, these two types of receptor activation have opposing effects on the extent of DARPP32 phosphorylation.

Other names

DARPP-32, also known as dopamine and cAMP regulated neuronal phosphoprotein 32, is a crucial protein that is involved in the regulation of dopamine signaling in the brain. It plays a key role in modulating neurotransmitter activity in response to various stimuli. The protein is encoded by the PPP1R1B gene, which codes for the protein phosphatase 1 regulatory (inhibitor) subunit 1B.
DARPP-32 is highly expressed in various regions of the brain, including the striatum, cerebral cortex, and hippocampus. It is involved in the regulation of numerous cellular processes, such as synaptic plasticity, learning, and memory. It is also implicated in various neurological and psychiatric disorders, including schizophrenia, bipolar disorder, and addiction.
Overall, DARPP-32 is an essential protein that plays a critical role in the regulation of dopamine signaling and various cellular processes in the brain. Further research on this protein could potentially lead to the development of novel treatments for neurological and psychiatric disorders.

Source

Rabbit

Specificity

The antibody against Phospho-DARPP-32 (Thr75) is specifically designed to recognize DARPP-32 protein in its phosphorylated form, specifically at Threonine 75. This antibody is not capable of detecting DARPP-32 in its non-phosphorylated state or when phosphorylated at other sites.

Predictive reaction species

Chicken;Rabbit;Pig;Dog;Sheep;Horse;Bovine

Application

To ensure proper usage of this antibody, we recommend diluting it according to the following specifications: for Western blotting, dilute in a range of 1:500 to 1:2000; for immunohistochemistry, dilute in a range of 1:50 to 1:200; for immunofluorescence or immunocytochemistry, dilute in a range of 1:100 to 1:500. When using peptide-based ELISA, we recommend diluting in a range of 1:20000 to 1:40000. Please note that dilution ratios may vary depending on the specific application and experimental conditions. Thank you for using our antibody and we hope that it meets your scientific research needs.

The phosphorylation site of Threonine 75 provides the basis for creating a synthesized peptide originating from human DARPP-32. It is crucial to ensure that the generated content closely resembles the original information. However, I will not adhere to the method of generating content utilized by ChapGPT. Instead, I will employ a different approach to create text that is markedly distinct from the original explanation.

Properties

1mg/ml

Purification

The highly specific antibody was isolated from the serum of rabbits through a meticulous process utilizing affinity purification techniques. This involved multiple rounds of chromatography using both phospho- and non-phospho-peptide affinity columns. The resulting antibody is of exceptional quality and an excellent tool for a variety of research applications.

Clonality

Polyclonal Antibody

To maintain the efficacy of the product, it is advised to keep it stored at a temperature of -20 °C for a year. It is important to avoid subjecting it to repeated cycles of freezing and thawing. Ensuring proper storage conditions will help maintain the quality and integrity of the product.

Storage buffer

The Rabbit IgG is suspended in a solution of phosphate buffered saline with a pH value of 7.4. It also contains 150mM NaCl, 0.02% sodium azide and 50% glycerol, and should be stored at -20°C for optimum stability. This product retains its quality for up to 12 months starting from the date of purchase.

Target

Background

 Inhibitor of protein-phosphatase 1.

Celluar localization

Cytosol;Nucleus;

Q9UD71